RYK
RYK (receptor like tyrosine kinase) is an atypical, catalytically inactive member of the receptor tyrosine kinase family that acts in Wnt signalling. It is also known as D3S3195, RYK1, JTK5 and JTK5A. Browse Assay Genie's current range of RYK research reagents below, or read on for the biology behind the target.
RYK may act as a coreceptor, together with FZD8, for Wnt proteins such as WNT1, WNT3, WNT3A and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth, and it stimulates Wnt signalling pathways such as the regulation of axon pathfinding. After WNT3 stimulation, the receptor is cleaved within its transmembrane region. This allows the C-terminal intracellular product to move from the cytoplasm to the nucleus, where it plays a crucial role in neuronal development.
The RYK protein has a leucine-rich extracellular domain containing a WIF-type Wnt binding region, a single transmembrane domain and an intracellular tyrosine kinase domain. It belongs to the protein kinase superfamily and the Tyr protein kinase family. It is atypical, however, because it differs from other growth factor receptor tyrosine kinases at several conserved residues in the activation and nucleotide binding domains. It is annotated as inactive, and members of its subfamily do not appear to be regulated by phosphorylation in the activation segment. One suggestion is that it acts by recruiting a signalling-competent auxiliary protein, through a mechanism that is not yet characterised. UniProt lists localisation to the cell membrane, nucleus and cytoplasm.
HGNC places the RYK gene in the pseudokinase group and, more specifically, among the WNT binding RTK type pseudokinases. Previous names include JTK5A protein tyrosine kinase and RYK receptor-like tyrosine kinase. Alternative splicing of the gene produces multiple transcript variants that encode distinct isoforms. The mouse ortholog is Ryk.
RYK contains a WIF-type Wnt binding region and may act with FZD8 as a coreceptor for WNT1, WNT3, WNT3A and WNT5A.
RYK stimulates Wnt signalling pathways involved in the regulation of axon pathfinding and neurite outgrowth.
After WNT3 stimulation, the cleaved C-terminal fragment of RYK enters the nucleus and plays a crucial role in neuronal development.
RYK is an atypical receptor tyrosine kinase that differs from related kinases at conserved residues in its activation and nucleotide binding domains.
RYK may act as a coreceptor with FZD8 for Wnt proteins including WNT1, WNT3, WNT3A and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth, and it stimulates Wnt signalling pathways that regulate axon pathfinding.
RYK is also called D3S3195, RYK1, JTK5 and JTK5A. Earlier names were JTK5A protein tyrosine kinase and RYK receptor-like tyrosine kinase. UniProt lists it as inactive tyrosine-protein kinase RYK. Alternative splicing of the gene gives multiple transcript variants that encode distinct isoforms.
No. RYK is an atypical member of the growth factor receptor tyrosine kinase family. It differs from other members at conserved residues in the activation and nucleotide binding domains, and HGNC classes it as a pseudokinase. A mouse ortholog, Ryk, is recorded. Check the datasheet of any product for its validated reactivity.
Wnt ligands, Frizzled receptors and other components of Wnt signalling that are linked to RYK function.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P34925; product specifications from Assay Genie datasheets.