MSRB3
MSRB3 (methionine sulfoxide reductase B3) is a zinc-dependent enzyme that reduces methionine sulfoxide back to methionine. It is also known as methionine-R-sulfoxide reductase B3, DFNB74, FLJ36866 and DKFZp686C1178, and it belongs to the MsrB family. Browse Assay Genie's current range of MSRB3 research reagents below, or read on for the biology behind the target.
MSRB3 catalyses the reduction of both free and protein-bound methionine sulfoxide to methionine. According to the NCBI Gene summary, the enzyme acts as a monomer and requires zinc as a cofactor. It is classed with the methionine sulfoxide reductases and belongs to the MsrB Met sulfoxide reductase family, which also includes MSRB2. UniProt lists two EC numbers for the enzyme, 1.8.4.12 and 1.8.4.14.
The MSRB3 gene is found at 12q14.3. Several transcript variants encode two different isoforms, and these differ in where they sit in the cell. One isoform localises to mitochondria and the other to the endoplasmic reticulum. This split is the basis of the two subcellular locations recorded for the MSRB3 protein. Researchers who study the MSRB3 gene or its product usually need to keep the isoforms apart.
The isoforms also differ in function. UniProt reports that isoform 2 is essential for hearing. This fits the previous HGNC gene name, deafness, autosomal recessive 74, and the alias DFNB74. The mouse ortholog is Msrb3, which allows the enzyme to be studied in a model organism. Work on MSRB3, whether by MSRB3 antibody or MSRB3 ELISA kit based methods, therefore bears on redox-related protein repair and on hearing biology.
MSRB3 reduces protein-bound methionine sulfoxide to methionine, making it a useful enzyme for studying how oxidised methionine residues are reversed.
Isoform 2 is essential for hearing, and the gene carries the former name deafness, autosomal recessive 74 (DFNB74).
One isoform localises to mitochondria and the other to the endoplasmic reticulum, allowing comparison of compartment-specific methionine sulfoxide reduction.
MSRB3 acts as a zinc-dependent monomer within the MsrB family, so it suits comparative work with other methionine sulfoxide reductases.
MSRB3 catalyses the reduction of free and protein-bound methionine sulfoxide to methionine. The NCBI Gene summary states that it acts as a monomer and needs zinc as a cofactor. UniProt also reports that isoform 2 is essential for hearing.
MSRB3 is also called methionine-R-sulfoxide reductase B3, DFNB74, FLJ36866 and DKFZp686C1178. Its earlier HGNC name was deafness, autosomal recessive 74. Transcript variants encode two isoforms, one localised to mitochondria and one to the endoplasmic reticulum.
The mouse ortholog is Msrb3. The gene's former name, deafness, autosomal recessive 74, and the UniProt note that isoform 2 is essential for hearing link MSRB3 to hearing. Check the datasheet of any product for its validated reactivity.
Other methionine sulfoxide reductases from the MsrB family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8IXL7; product specifications from Assay Genie datasheets.