PRDX6
PRDX6 (peroxiredoxin 6) is a bifunctional thiol-specific peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols. Also known as AOP2, 1-Cys, NSGPx and aiPLA2, it additionally has phospholipase and lysophospholipid acyltransferase activities. Browse Assay Genie's current range of PRDX6 research reagents below, or read on for the biology behind the target.
PRDX6 is a thiol-specific peroxidase that catalyses the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. It can act on H2O2 as well as short-chain organic, fatty acid and phospholipid hydroperoxides. Beyond its peroxidase role, the protein has phospholipase activity: it can either reduce the oxidised sn-2 fatty acyl group of a phospholipid or hydrolyse the sn-2 ester bond. Both activities depend on binding to phospholipids at acidic pH, and to oxidised phospholipids at cytosolic pH.
The PRDX6 protein also shows acyl-CoA-dependent lysophospholipid acyltransferase activity, converting lysophosphatidylcholine into phosphatidylcholine. It shows a clear preference for lysophosphatidylcholine as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate. Through these functions it contributes to cell protection against oxidative stress by detoxifying peroxides, and to phospholipid homeostasis. NCBI describes it as a bifunctional enzyme with two distinct active sites, involved in redox regulation of the cell and possibly in the regulation of phospholipid turnover and protection against oxidative injury.
The PRDX6 gene encodes a member of the thiol-specific antioxidant protein family. UniProt places the protein in the peroxiredoxin family, Prx6 subfamily, and HGNC classifies it in the peroxiredoxin and acyltransferase gene groups. It is found in the cytoplasm and in the lysosome. Literature and databases use several alternative names for the same protein, including AOP2, KIAA0106, 1-Cys, NSGPx, PRX, aiPLA2 and p29. The rat ortholog is also named Prdx6.
PRDX6 detoxifies hydrogen peroxide and organic hydroperoxides, making it a focus for studies of cell protection against oxidative stress and injury.
As a thiol-specific peroxidase involved in redox regulation of the cell, PRDX6 is studied alongside other peroxiredoxins to dissect peroxide handling.
Phospholipase and lysophosphatidylcholine acyltransferase activities link PRDX6 to phospholipid homeostasis and possibly phospholipid turnover.
PRDX6 can reduce fatty acid and phospholipid hydroperoxides, supporting work on how oxidised phospholipids are processed in cells.
PRDX6 is a thiol-specific peroxidase that reduces hydrogen peroxide and short-chain, fatty acid and phospholipid hydroperoxides. It also has phospholipase activity and acyl-CoA-dependent lysophospholipid acyltransferase activity, so it contributes to both protection against oxidative stress and phospholipid homeostasis.
PRDX6 (peroxiredoxin 6) is also called AOP2, KIAA0106, 1-Cys, NSGPx, PRX, aiPLA2, MGC46173 and p29. The names 1-Cys and aiPLA2 reflect its peroxidase and phospholipase activities. UniProt lists the protein as Peroxiredoxin-6, EC 1.11.1.27.
NCBI describes PRDX6 as a bifunctional enzyme with two distinct active sites: one gives peroxidase activity against hydroperoxides, the other gives phospholipase activity. Its lysophospholipid acyltransferase function is also annotated. The rat ortholog is Prdx6; check the datasheet of any product for its validated reactivity.
Other peroxiredoxin family members and peroxide-detoxifying enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P30041; product specifications from Assay Genie datasheets.