TCF7
TCF7 (transcription factor 7, also known as TCF-1) is a nuclear HMG-box transcriptional activator of the TCF/LEF family. It drives T-cell lymphocyte differentiation and acts with beta-catenin in Wnt signalling. Browse Assay Genie's current range of TCF7 research reagents below, or read on for the biology behind the target.
TCF7 is a transcriptional activator involved in T-cell lymphocyte differentiation. It is necessary for the survival of CD4(+) CD8(+) immature thymocytes, and it binds the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. In alpha-beta T-cell lineages it represses expression of the T-cell receptor gamma gene, and it is also required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells.
The TCF7 gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. The protein is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. It forms a complex with beta-catenin (CTNNB1) and activates transcription through the Wnt/beta-catenin signalling pathway. The TCF7 protein is also classed within the Wnt enhanceosome complex gene group. Alternative splicing gives multiple transcript variants. Isoforms lacking the N-terminal beta-catenin interaction domain cannot support thymocyte survival and may act as dominant negative regulators of Wnt signalling.
Regulation of TCF7 activity involves the co-repressors TLE1, TLE2, TLE3 and TLE4, which repress transactivation mediated by TCF7 and CTNNB1. TCF7 may also act as a feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. Mice with a knockout of the mouse ortholog Tcf7 are viable and fertile but show a block in T-lymphocyte differentiation. The gene was previously named transcription factor 7 (T-cell specific, HMG-box), and TCF-1 remains a common alias.
TCF7 is necessary for survival of immature CD4(+) CD8(+) thymocytes, and Tcf7 knockout mice show a block in T-lymphocyte differentiation.
TCF7 complexes with beta-catenin to activate transcription, while isoforms lacking the beta-catenin binding domain may act as dominant negative regulators.
TCF7 plays a critical role in natural killer cell and innate lymphoid cell development, including lymphoid tissue inducer T-cells.
TCF7 binds the CD3E promoter enhancer element, represses the T-cell receptor gamma gene and is modulated by TLE co-repressors.
TCF7 is a nuclear transcriptional activator that supports T-cell lymphocyte differentiation. It is necessary for the survival of immature CD4(+) CD8(+) thymocytes, binds the CD3E promoter enhancer element and partners with beta-catenin in Wnt signalling. It is also needed for lymphoid tissue inducer T-cell development.
Yes. TCF-1 is an alias for TCF7, and the gene was formerly named transcription factor 7 (T-cell specific, HMG-box). Alternative splicing produces multiple transcript variants, including isoforms lacking the N-terminal beta-catenin binding domain, which may act as dominant negative regulators of Wnt signalling.
The mouse ortholog is Tcf7. Mice with a knockout of this gene are viable and fertile but display a block in T-lymphocyte differentiation, underlining its role in T-cell development. Check the datasheet of any product for its validated reactivity.
Other TCF family members, Wnt signalling partners and T-cell regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P36402; product specifications from Assay Genie datasheets.