UBA3
UBA3 (ubiquitin like modifier activating enzyme 3, also known as hUba3, NAE2 and UBE1C) is the catalytic subunit of the NEDD8-activating E1 enzyme. Together with its partner NAE1 it activates the ubiquitin-like protein NEDD8 and passes it on to the E2 enzyme UBE2M. Browse Assay Genie's current range of UBA3 research reagents below, or read on for the biology behind the target.
UBA3 is the catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. The enzyme activates NEDD8 in two steps. It first adenylates the C-terminal glycine of NEDD8 using ATP. It then links this residue to the side chain of the catalytic cysteine, which produces a NEDD8-UBA3 thioester and free AMP. Finally, the E1 transfers NEDD8 to the catalytic cysteine of the E2 enzyme UBE2M. UniProt also reports that UBA3 down-regulates steroid receptor activity and is necessary for cell cycle progression.
The UBA3 gene encodes a member of the E1 ubiquitin-activating enzyme family; UniProt places the protein in the UBA3 subfamily of the ubiquitin-activating E1 family. Ubiquitination generally involves three classes of enzyme: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBA3 works at the first step of the related NEDD8 pathway. According to the NCBI Gene summary, the enzyme associates with AppBp1, an amyloid beta precursor protein binding protein (the partner referred to as NAE1 in UniProt), to form a heterodimer. This complex activates NEDD8, a ubiquitin-like protein that regulates cell division, signalling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been described for the UBA3 gene.
HGNC classifies UBA3 in the group of ubiquitin like modifier activating enzymes. Earlier names include ubiquitin-activating enzyme E1C (homologous to yeast UBA3), which explains the alias UBE1C. Mouse and rat both carry an orthologue named Uba3, so the protein can be studied in common rodent models. Researchers looking at neddylation, E1 enzymology or cell cycle control will find UBA3 a central component of the pathway.
UBA3 forms the NEDD8-specific E1 that adenylates NEDD8 and passes it to UBE2M, making it the entry point of the pathway.
As a member of the ubiquitin-activating E1 family, UBA3 is a model for studying ATP-dependent adenylation and thioester formation.
UBA3 is necessary for cell cycle progression, and NEDD8 activation regulates cell division and embryogenesis.
UniProt reports that UBA3 down-regulates steroid receptor activity, linking the enzyme to receptor signalling studies.
UBA3 is the catalytic subunit of the UBA3-NAE1 E1 enzyme. It adenylates the C-terminal glycine of NEDD8 with ATP, forms a NEDD8-UBA3 thioester, then transfers NEDD8 to the E2 enzyme UBE2M. It is also necessary for cell cycle progression.
UBA3 is also called hUba3, NAE2 and UBE1C, and its protein name is NEDD8-activating enzyme E1 catalytic subunit. Older HGNC names include ubiquitin-activating enzyme E1C (homologous to yeast UBA3). Several alternatively spliced transcript variants encode distinct isoforms.
Yes. Both mouse and rat have an orthologue named Uba3, so rodent models can be used to study the gene. Cross-reactivity differs between reagents, so check the datasheet of any product for its validated reactivity.
Other ubiquitin-like modifier activating enzymes and related ubiquitin pathway targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TBC4; product specifications from Assay Genie datasheets.