UBE2K
UBE2K (ubiquitin conjugating enzyme E2 K, also known as HIP2, HYPG and UBC1) is an E2 enzyme that accepts ubiquitin from the E1 complex and catalyses its attachment to other proteins. It is best known for ubiquitinating huntingtin and for building 'Lys-48'-linked polyubiquitin chains. Browse Assay Genie's current range of UBE2K research reagents below, or read on for the biology behind the target.
UBE2K acts in the middle of the ubiquitin cascade. It accepts ubiquitin from the E1 complex and catalyses its covalent attachment to other proteins. In vitro, with or without the BRCA1-BARD1 E3 ubiquitin-protein ligase complex, it synthesises 'Lys-48'-linked polyubiquitin chains. It does not transfer ubiquitin directly to a substrate. Instead it elongates a substrate that is already monoubiquitinated. Through this activity it mediates the selective degradation of short-lived and abnormal proteins, including endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins.
The UBE2K gene encodes a member of the ubiquitin-conjugating enzyme family, grouped by HGNC with the E2 ubiquitin conjugating enzymes. The protein is found in the cytoplasm and interacts with RING finger proteins. Multiple transcript variants encoding different isoforms have been identified. The mouse ortholog is Ube2k. Researchers studying this enzyme may use a UBE2K antibody or UBE2K ELISA kit to follow its expression, and UBE2K protein in ubiquitination assays.
The name HIP2 (huntingtin interacting protein 2) reflects its ability to ubiquitinate huntingtin, the gene product for Huntington's disease. Reported functions include a role in aggregate formation of expanded polyglutamine proteins and in suppressing apoptosis in polyglutamine diseases. UBE2K is also linked to the dislocation of newly synthesised MHC class I heavy chains from the endoplasmic reticulum. It may be involved in the US11-dependent degradation of these chains during cytomegalovirus infection, and in HPV E7-dependent degradation of RB1. It may contribute to foam cell formation by promoting degradation of p53/TP53, which suppresses apoptosis in lipid-bearing macrophages. It has also been proposed to act in ubiquitination and proteolytic processing of NF-kappa-B, and supports ubiquitination of NFKB1 in vitro.
UBE2K ubiquitinates huntingtin and has a role in aggregate formation of expanded polyglutamine proteins and in suppressing apoptosis in polyglutamine diseases.
It mediates selective degradation of short-lived and abnormal proteins, including ERAD of misfolded lumenal proteins, through 'Lys-48'-linked polyubiquitin chains.
UBE2K may be involved in US11-dependent degradation of MHC class I heavy chains in cytomegalovirus infection, and in HPV E7-dependent degradation of RB1.
It may mediate foam cell formation by suppressing apoptosis of lipid-bearing macrophages through ubiquitination and degradation of p53/TP53.
UBE2K is an E2 enzyme that accepts ubiquitin from the E1 complex and catalyses its attachment to other proteins. It elongates monoubiquitinated substrates with 'Lys-48'-linked chains rather than transferring ubiquitin directly, and so mediates degradation of short-lived and misfolded proteins.
UBE2K is also called HIP2, HYPG and UBC1. Earlier names include huntingtin interacting protein 2 and ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast). Multiple transcript variants encoding different isoforms have been identified for the gene.
UBE2K ubiquitinates huntingtin, the gene product for Huntington's disease, and has roles in polyglutamine aggregate formation and apoptosis suppression. A mouse ortholog, Ube2k, exists. Check the datasheet of any product for its validated reactivity.
Other ubiquitin-conjugating E2 enzymes and ubiquitin ligases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P61086; product specifications from Assay Genie datasheets.