RCHY1
RCHY1 (ring finger and CHY zinc finger domain containing 1) is a RING finger protein reported to act as an E3 ubiquitin ligase, targeting proteins for proteasomal degradation. It is also known as PIRH2, ARNIP, CHIMP, ZCHY, RNF199 and ZNF363. Browse Assay Genie's current range of RCHY1 research reagents below, or read on for the biology behind the target.
RCHY1 is described in the NCBI Gene summary as a protein with ubiquitin ligase activity. It is reported to mediate E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumour protein p53, histone deacetylase 1 (HDAC1) and cyclin-dependent kinase inhibitor 1B (p27Kip1). By controlling the levels of these substrates, RCHY1 is linked to the regulation of cell cycle progression. The UniProt function annotation, however, carries a note that the protein has no E3 ubiquitin-protein ligase activity, so the enzymatic role of RCHY1 should be read with this difference between sources in mind.
The RCHY1 gene encodes a RING finger and CHY zinc finger domain-containing protein. HGNC places it in the groups of ring finger proteins and RING finger E3 ubiquitin protein ligases, and the enzyme classification EC 2.3.2.27 is associated with it. UniProt records its subcellular locations as the nucleus, nuclear speckles and the cytoplasm. Alternatively spliced transcript variants that encode different isoforms have been described for this gene. The mouse ortholog is Rchy1.
The gene has carried several names over time. ZNF363 is the previous HGNC symbol, and the aliases PIRH2, ARNIP, CHIMP, ZCHY, RNF199, PRO1996 and DKFZp586C1620 appear in the literature and databases. Researchers studying the RCHY1 protein usually do so in the context of the ubiquitin-proteasome system, p53 turnover and cell cycle control, where an RCHY1 gene product that affects several regulators is of interest.
RCHY1 is classed as a RING finger E3 ubiquitin protein ligase, making it a subject for studies of substrate ubiquitination and proteasomal degradation.
RCHY1 is reported to mediate ubiquitination and degradation of tumour protein p53, so it is relevant to work on how p53 levels are controlled.
By affecting levels of p27Kip1 and HDAC1, RCHY1 is linked to the regulation of cell cycle progression.
UniProt places RCHY1 in the nucleus, nuclear speckles and cytoplasm, which supports studies of its localisation and compartment-specific functions.
According to the NCBI Gene summary, RCHY1 mediates E3-dependent ubiquitination and proteasomal degradation of targets including p53, HDAC1 and p27Kip1, thereby regulating their levels and cell cycle progression. UniProt's function annotation notes that it has no E3 ubiquitin-protein ligase activity, so the two sources differ.
RCHY1 is also called PIRH2, ARNIP, CHIMP, ZCHY, RNF199, PRO1996 and DKFZp586C1620. ZNF363 is its previous HGNC symbol. The full name is ring finger and CHY zinc finger domain containing 1. Alternatively spliced transcript variants encoding different isoforms have been described.
The mouse ortholog is Rchy1. The data supplied here do not list a rat ortholog, so check the datasheet of any product for its validated reactivity before choosing reagents for a given species.
Reported RCHY1 substrates, p53 pathway regulators and other ubiquitin-system proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96PM5; product specifications from Assay Genie datasheets.