TOP2B
TOP2B (DNA topoisomerase II beta, also known as top2beta and TOPIIB) is a nuclear type II topoisomerase that alters DNA topology by passing one double-stranded DNA segment through a transient break in another. It acts as a key decatenating enzyme and plays a role in B-cell differentiation. Browse Assay Genie's current range of TOP2B research reagents below, or read on for the biology behind the target.
TOP2B is a DNA topoisomerase that controls and alters the topological state of DNA during transcription. It binds two double-stranded DNA molecules, generates a double-stranded break in one of them, passes the intact strand through the break and then religates the broken strand. This transient breaking and rejoining of duplex DNA makes TOP2B a key decatenating enzyme. The nuclear enzyme is involved in chromosome condensation, chromatid separation and the relief of torsional stress that arises during DNA transcription and replication. UniProt also assigns it a role in B-cell differentiation.
The protein belongs to the type II topoisomerase family and is classed by HGNC in the topoisomerases gene group. It localises to the nucleus, with annotation to both the nucleoplasm and the nucleolus. The TOP2B gene is found on chromosome 3, and alternative splicing results in multiple transcript variants. Two forms of type II topoisomerase exist, probably as products of a gene duplication event: the beta form encoded by TOP2B and the alpha form, TOP2A, which is encoded on chromosome 17. Researchers studying either form can compare TOP2B with TOP2A, and with the type I and type III enzymes TOP1 and TOP3A.
TOP2B has attracted attention in disease research. The gene encodes a target of several anticancer agents, and a variety of mutations in it have been associated with the development of drug resistance. Reduced activity of the enzyme may also play a role in ataxia-telangiectasia. HGNC previously listed the gene as topoisomerase (DNA) II beta 180kDa, and TOP2B antibody, TOP2B ELISA kit and TOP2B protein tools are commonly used to examine its expression and function.
TOP2B alters DNA topology during transcription and relieves the torsional stress generated during transcription and replication.
The enzyme is involved in chromosome condensation and chromatid separation, and acts as a key decatenating enzyme.
TOP2B is a target for several anticancer agents, and mutations in its gene have been associated with drug resistance.
UniProt annotates TOP2B as playing a role in B-cell differentiation, and reduced activity may contribute to ataxia-telangiectasia.
TOP2B is a type II topoisomerase and key decatenating enzyme. It binds two double-stranded DNA molecules, breaks one, passes the intact strand through the break and religates it. This alters DNA topology during transcription and replication, and the enzyme also plays a role in B-cell differentiation.
TOP2B is also known as top2beta and TOPIIB. Its HGNC name is DNA topoisomerase II beta, UniProt calls it DNA topoisomerase 2-beta, and an earlier HGNC name was topoisomerase (DNA) II beta 180kDa. Alternative splicing produces multiple transcript variants.
TOP2B and TOP2A are the beta and alpha forms of type II topoisomerase, likely arising from a gene duplication event. The mouse ortholog is Top2b. TOP2B is an anticancer drug target, and reduced activity may play a role in ataxia-telangiectasia. Check the datasheet of any product for its validated reactivity.
Other human DNA topoisomerases, including the alpha form of type II topoisomerase, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q02880; product specifications from Assay Genie datasheets.