SNRPA
SNRPA (small nuclear ribonucleoprotein polypeptide A, also known as U1A, U1-A or Mud1) is a component of the spliceosomal U1 snRNP. It binds stem loop II of U1 snRNA and contributes to recognition of the pre-mRNA 5' splice site. Browse Assay Genie's current range of SNRPA research reagents below, or read on for the biology behind the target.
SNRPA is a component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA, and this interaction is required for the later binding of U2 snRNP and the U4/U6/U5 tri-snRNP. Within U1 snRNP, SNRPA binds stem loop II of U1 snRNA. It may also bind preferentially to the 5'-UGCAC-3' motif on RNAs.
Outside the snRNP, SNRPA can exist in a snRNP-free form known as SF-A, which may be involved in coupled pre-mRNA splicing and polyadenylation. The NCBI Gene summary notes that the encoded protein autoregulates itself by inhibiting polyadenylation of its own pre-mRNA via dimerisation, and that it has been implicated in coupling splicing to polyadenylation. This makes the SNRPA gene a useful example of feedback control at the level of RNA processing.
SNRPA belongs to the RRM U1 A/B'' family and is localised to the nucleus. HGNC places it in the RNA binding motif containing and U1 small nuclear ribonucleoprotein gene groups. The protein is also referred to as U1 small nuclear ribonucleoprotein A, U1A, U1-A and Mud1, and the mouse ortholog is Snrpa. The related protein SNRPA1 is a separate gene product.
SNRPA is part of U1 snRNP, the first snRNP to engage pre-mRNA, so it is relevant to studies of 5' splice-site recognition.
U1 snRNP binding to pre-mRNA is required for subsequent recruitment of U2 snRNP and the U4/U6/U5 tri-snRNP.
The snRNP-free form SF-A may link pre-mRNA splicing with polyadenylation, a process of interest in RNA processing research.
SNRPA binds U1 snRNA stem loop II and may prefer the 5'-UGCAC-3' motif, making it a model RNA recognition motif protein.
SNRPA is a component of the spliceosomal U1 snRNP, which recognises the pre-mRNA 5' splice site and initiates spliceosome assembly. It binds stem loop II of U1 snRNA. In its snRNP-free form (SF-A) it may participate in coupled splicing and polyadenylation.
SNRPA stands for small nuclear ribonucleoprotein polypeptide A. Aliases include U1A, U1-A and Mud1, and UniProt names the protein U1 small nuclear ribonucleoprotein A. It is a member of the RRM U1 A/B'' family and is found in the nucleus.
According to the NCBI Gene summary, the protein autoregulates by inhibiting polyadenylation of its own pre-mRNA through dimerisation. This property is linked to its proposed role in coupling splicing and polyadenylation. The mouse ortholog is Snrpa; check the datasheet of any product for its validated reactivity.
Related spliceosomal and RNA-binding proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P09012; product specifications from Assay Genie datasheets.