CYB5A
CYB5A (cytochrome b5 type A, also known as MCB5 or CYB5) is a membrane-bound hemoprotein that acts as an electron carrier for several membrane-bound oxygenases. It also reduces methemoglobin, and defects in the gene are linked to hereditary methemoglobinemia. Browse Assay Genie's current range of CYB5A research reagents below, or read on for the biology behind the target.
CYB5A is a membrane-bound hemoprotein that functions as an electron carrier for several membrane-bound oxygenases. The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, an activity that is required for stearyl-CoA-desaturase activity. In this way cytochrome b5 links electron transfer to both haemoglobin redox state and fatty acid desaturation.
The CYB5A gene encodes cytochrome b5, a member of the cytochrome b5 family and of the HGNC group of cytochrome b5 domain containing genes. UniProt records the protein in the endoplasmic reticulum membrane, the microsome membrane and the cytoplasm. Three transcript variants encoding different isoforms have been described for the gene. Mouse and rat each have a Cyb5a ortholog, which allows the biology to be studied in common model species.
The gene has appeared in the literature under several names, including MCB5 and CYB5, and earlier descriptions include cytochrome b-5, cytochrome b5 (microsomal) and cytochrome b5 type A (microsomal). Defects in CYB5A are a cause of type IV hereditary methemoglobinemia, making the protein relevant to studies of haemoglobin redox control. Researchers working with a CYB5A antibody, CYB5A ELISA kit or recombinant CYB5A protein often examine it alongside cytochrome b5 reductases, which are related redox partners in the same area of biology.
CYB5A reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, making it a focus for studies of haemoglobin redox state.
Defects in the CYB5A gene cause type IV hereditary methemoglobinemia, so the protein is studied in this inherited disorder.
The reduction of haemoglobin by cytochrome b5 is required for stearyl-CoA-desaturase activity, linking CYB5A to fatty acid metabolism research.
As an electron carrier for several membrane-bound oxygenases, CYB5A is used to explore electron transfer at endoplasmic reticulum membranes.
CYB5A is a membrane-bound hemoprotein that works as an electron carrier for several membrane-bound oxygenases. It reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity.
The gene is also known as MCB5 and CYB5, and the protein as cytochrome b5. Older names include cytochrome b-5 and cytochrome b5 (microsomal). Three transcript variants encoding different isoforms have been reported for the gene.
Defects in CYB5A are a cause of type IV hereditary methemoglobinemia. Mouse and rat both have a Cyb5a ortholog, so rodent models can be used; check the datasheet of any product for its validated reactivity.
Other cytochrome b5 reductases and cytochrome b family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P00167; product specifications from Assay Genie datasheets.