RPS6KA5
RPS6KA5 (ribosomal protein S6 kinase A5), also known as MSK1 or RLPK, is a serine/threonine-protein kinase of the AGC family. It phosphorylates transcription factors and histones in response to mitogen and stress signals, linking upstream kinase cascades to gene expression. Browse Assay Genie's current range of RPS6KA5 research reagents below, or read on for the biology behind the target.
RPS6KA5 is required for the mitogen- or stress-induced phosphorylation of the transcription factors CREB1 and ATF1, and it regulates RELA, STAT3 and ETV1/ER81. It responds to stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. It also contributes to gene activation through histone phosphorylation: it phosphorylates histone H3 at Ser-10, which drives transcription of immediate early genes including FOS and JUN. It can repress transcription directly by phosphorylating Ser-1 of histone H2A. It also mediates phosphorylation of HMGN1/HMG14.
The kinase has a prominent role in inflammatory gene control. It associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory genes. In lipopolysaccharide-stimulated primary macrophages it acts downstream of TLR4 to limit pro-inflammatory cytokine production, probably by inducing DUSP1 and interleukin 10 through CREB1 and ATF1. In erythropoietin-stimulated cells it is needed for STAT3 Ser-727 phosphorylation. It phosphorylates TRIM7 at Ser-107 downstream of MEK/ERK signalling, which stimulates TRIM7 ubiquitin ligase activity. It also mediates the downstream effects of excitotoxic injury in neuronal cell death.
The RPS6KA5 gene encodes a member of the S6 kinase subfamily of the AGC protein kinase family. HGNC places it in the MAPK activated protein kinases group and the ribosomal protein S6 kinase family, and its earlier name was ribosomal protein S6 kinase, 90kD, polypeptide 5. NCBI Gene describes it as enabling ATP binding and protein kinase activity, involved in interleukin-1-mediated signalling, and located in the cytoplasm and nucleoplasm. The mouse ortholog is Rps6ka5. Researchers studying MSK1 may look for an RPS6KA5 antibody or RPS6KA5 protein reagent for work on this signalling axis.
RPS6KA5 limits pro-inflammatory cytokine production downstream of TLR4 and contributes to repression of inflammatory genes with the glucocorticoid receptor.
It phosphorylates histone H3 and H2A and transcription factors such as CREB1, ATF1 and RELA, making it a model for signal-to-chromatin regulation.
The kinase acts in mitogen and stress responses, including EGF signalling, and phosphorylates TRIM7 downstream of the MEK/ERK pathway.
RPS6KA5 mediates downstream effects of excitotoxic injury in neurons and regulates ETV1/ER81, which may matter in breast tumour formation.
RPS6KA5 is a serine/threonine kinase that phosphorylates CREB1, ATF1, RELA, STAT3, ETV1/ER81 and histones H3 and H2A in response to mitogen or stress signals. Through these targets it activates immediate early genes and restrains inflammatory gene expression.
RPS6KA5 is also called MSK1 and RLPK. Its UniProt name is ribosomal protein S6 kinase alpha-5, and an earlier HGNC name was ribosomal protein S6 kinase, 90kD, polypeptide 5. It belongs to the AGC kinase family, S6 kinase subfamily.
A mouse ortholog, Rps6ka5, is recorded for this gene, so rodent models can be used to study its function. The kinase has roles in macrophage inflammation and neuronal cell death. Check the datasheet of any product for its validated reactivity.
Other ribosomal S6 kinase family members and related ribosomal protein targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O75582; product specifications from Assay Genie datasheets.