Western blot analysis of various lysates, using RPS6KA5 Rabbit mAb (CAB23891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The RPS6KA5 Monoclonal Antibody (CAB23891) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced via hybridoma technology, is specifically designed for use in various experimental applications, such as immunofluorescence and flow cytometry.RPS6KA5, also known as ribosomal protein S6 kinase A5 or MSK1, plays a crucial role in intracellular signaling pathways that regulate gene expression and cell behavior in response to various stimuli.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
RPS6KA5 Monoclonal Antibody
SKU:
CAB23891
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Monkey
Clone Number:
ARC61540
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MSK1, RLPK, MSPK1, RPS6KA5
Positive Sample:
SK-BR-3, C6, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
62kDa/82kDa/90kDa
Observed MW:
80kDa/100kDa/80kDa/90kDa
Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
Purification Method
Affinity purification
Gene ID
9252
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RPS6KA5 Rabbit mAb (CAB23891) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse brain using RPS6KA5 Rabbit mAb (CAB23891) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
