TNKS2
TNKS2 (tankyrase 2, also known as PARP-5b, PARP-5c, ARTD6 or TANK2) is a poly-ADP-ribosyltransferase of the ARTD/PARP family. It modifies target proteins with poly-ADP-ribose and is involved in Wnt signalling, telomere length regulation and vesicle trafficking. Browse Assay Genie's current range of TNKS2 research reagents below, or read on for the biology behind the target.
TNKS2 is a poly-ADP-ribosyltransferase that uses NAD+ to attach poly-ADP-ribose chains to substrate proteins. It acts as an activator of the Wnt signalling pathway by poly-ADP-ribosylating AXIN1 and AXIN2, two key components of the beta-catenin destruction complex. The modified proteins are recognised by the E3 ligase RNF146, which ubiquitinates them and targets them for degradation. The same mechanism applies to BLZF1 and CASC3, which are poly-ADP-ribosylated and then recruit RNF146 for ubiquitination. TNKS2 also stimulates 26S proteasome activity.
In telomere biology, TNKS2 poly-ADP-ribosylates TERF1 and so contributes to the regulation of telomere length. This fits its annotated localisation, which includes the telomere as well as the cytoplasm, Golgi apparatus membrane and nucleus. NCBI Gene also places it at the nuclear envelope, pericentriolar material and perinuclear region of the cytoplasm, and lists both NAD+ poly-ADP-ribosyltransferase and NAD+-protein mono-ADP-ribosyltransferase activities, as well as enzyme binding. The TNKS2 gene is annotated as a positive regulator of canonical Wnt signalling and as involved in post-translational protein modification and telomere maintenance.
TNKS2 belongs to the ARTD/PARP family and is a member of the HGNC groups for ankyrin repeat domain containing proteins, poly(ADP-ribose) polymerases and sterile alpha motif domain containing proteins. Its earlier names were tankyrase and TRF1-interacting ankyrin-related ADP-ribose polymerase 2, and other symbols include TNKL, PARP5B, PARP5C and pART6. The mouse ortholog is Tnks2. The paralogue TNKS is a closely related tankyrase.
TNKS2 poly-ADP-ribosylates AXIN1 and AXIN2, leading to their RNF146-mediated degradation, so it is studied as a regulator of the beta-catenin destruction complex.
By poly-ADP-ribosylating TERF1, TNKS2 contributes to the regulation of telomere length and is relevant to telomere maintenance studies.
TNKS2 substrates are ubiquitinated after recruitment of RNF146, and TNKS2 stimulates 26S proteasome activity, linking ADP-ribosylation to protein turnover.
As an ARTD/PARP family member with poly- and mono-ADP-ribosyltransferase activity, TNKS2 is used to explore post-translational modification by NAD+.
TNKS2 is a poly-ADP-ribosyltransferase. It modifies AXIN1 and AXIN2, which are then ubiquitinated through RNF146 and degraded, thereby activating Wnt signalling. It also modifies BLZF1, CASC3 and TERF1, the last contributing to telomere length regulation, and it stimulates 26S proteasome activity.
TNKS2 is the symbol for tankyrase 2. Aliases include TNKL, TANK2, PARP-5b, PARP-5c, PARP5B, PARP5C, pART6 and ARTD6. Previous names are tankyrase and TRF1-interacting ankyrin-related ADP-ribose polymerase 2. UniProt calls it Poly [ADP-ribose] polymerase tankyrase-2.
The mouse ortholog is Tnks2, so mouse models can be used to study it. TNKS2 belongs to the ARTD/PARP family and is related to the paralogue TNKS, another tankyrase. Check the datasheet of any product for its validated reactivity.
Tankyrase paralogues and related targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H2K2; product specifications from Assay Genie datasheets.