NFYB
NFYB (nuclear transcription factor Y subunit beta, also known as NF-YB, CBF-A and HAP3) is one subunit of the heterotrimeric NF-Y transcription factor. NF-Y recognises the CCAAT box motif in the promoters of its target genes. Browse Assay Genie's current range of NFYB research reagents below, or read on for the biology behind the target.
NFYB is a component of NF-Y, a sequence-specific heterotrimeric transcription factor that specifically recognises a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can act as both an activator and a repressor, depending on the cofactors it interacts with. The complex is highly conserved and binds CCAAT motifs in the promoter regions of a variety of genes.
The NFYB protein is subunit B of the trimer. It forms a tight dimer with the C subunit, and this dimer is a prerequisite for association of subunit A. The resulting trimer binds DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif, and the histone nature of these subunits is supported by protein sequence alignments and by experiments with mutants. UniProt places NFYB in the NFYB/HAP3 subunit family, and the protein localises to the nucleus.
The human NFYB gene is listed by HGNC within the nuclear transcription factor Y gene group. Previous names and aliases include nuclear transcription factor Y, beta, CBF-A, HAP3 and NF-YB. Mouse and rat both carry an ortholog named Nfyb, so the gene and its NFYB protein can be studied in common rodent models as well as in human systems.
NFYB is part of NF-Y, which binds CCAAT motifs in promoters and can activate or repress target genes depending on its cofactors.
As a subunit of a factor that binds CCAAT motifs with high specificity and affinity, NFYB is relevant to analysis of promoter architecture.
NFYB dimerises tightly with subunit C, a prerequisite for subunit A association, making it a model for stepwise trimeric complex formation.
Subunits B and C contain histone-like motifs supported by sequence alignments and mutant experiments, linking NFYB to studies of histone-fold DNA binding.
NFYB is subunit B of the heterotrimeric transcription factor NF-Y, which recognises the 5'-CCAAT-3' box in target gene promoters. It dimerises tightly with subunit C, and this dimer allows subunit A to join. NF-Y can activate or repress transcription depending on its cofactors.
NFYB is also called NF-YB, CBF-A and HAP3. Its HGNC full name is nuclear transcription factor Y subunit beta, and a previous name was nuclear transcription factor Y, beta. UniProt classifies it in the NFYB/HAP3 subunit family.
The NF-Y complex is described as a highly conserved transcription factor. Orthologs of the human NFYB gene are named Nfyb in both mouse and rat. Check the datasheet of any product for its validated reactivity.
Other subunits of the NF-Y complex and CCAAT-box transcription factors are listed here where pages exist.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P25208; product specifications from Assay Genie datasheets.