NFYB Transcription Factor Activity Assay



ELISA Kit Technical Manual

NFYB Transcription Factor activity assay

Product Description:The Assay Genie NFYB transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Assay Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:CAAT box transcription factor activity protein subunit B;Nuclear transcription factor Y subunit B Short name: NF-YB
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse, Rat
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

NFYB Transcription Factor activity assay Protein Information

UniProt Protein Function:NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process

NCBI Summary:The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
UniProt Code:P25208
NCBI GenInfo Identifier:399193
NCBI Gene ID:4801
NCBI Accession:P25208.2
UniProt Secondary Accession:P25208,Q96IY8, A8K7B9,
UniProt Related Accession:P25208
Molecular Weight:207
NCBI Full Name:Nuclear transcription factor Y subunit beta
NCBI Synonym Full Names:nuclear transcription factor Y, beta
NCBI Official Symbol:NFYB
NCBI Official Synonym Symbols:HAP3; CBF-A; CBF-B; NF-YB
NCBI Protein Information:nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A
UniProt Protein Name:Nuclear transcription factor Y subunit beta
UniProt Synonym Protein Names:CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB
Protein Family:Nuclear transcription factor
UniProt Gene Name:NFYB
UniProt Entry Name:NFYB_HUMAN
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Additional Information

Product Type:
Transcription Factor Assay
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