Description
NFYB Transcription Factor Activity Assay
The NFYB (Nuclear Transcription Factor Y Subunit Beta) Transcription Factor Activity Assay Kit is designed for accurate measurement of NFYB transcription factor activity in various biological samples. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for research applications.NFYB is an important transcription factor involved in regulating gene expression and is known to play a crucial role in various cellular processes. Understanding NFYB activity can provide insights into gene regulation mechanisms and potential therapeutic targets in diseases such as cancer, autoimmune disorders, and developmental abnormalities.
The NFYB Transcription Factor Activity Assay Kit offers a versatile tool for studying NFYB function and exploring its role in different biological processes. It is suitable for researchers looking to investigate transcription regulation, gene expression, and potential therapeutic interventions targeting NFYB.
| Product Name: | NFYB Transcription Factor Activity Assay |
| Product Code: | TFAB00188 |
| Target: | NFYB |
| Synonyms: | CAAT box transcription factor activity protein subunit B, Nuclear transcription factor Y subunit B, NF-YB |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NFYB transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 12q22-q23 Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process |
| NCBI Summary: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25208 |
| NCBI GenInfo Identifier: | 399193 |
| NCBI Gene ID: | 4801 |
| NCBI Accession: | P25208.2 |
| UniProt Secondary Accession: | P25208,Q96IY8, A8K7B9, |
| UniProt Related Accession: | P25208 |
| Molecular Weight: | 207 |
| NCBI Full Name: | Nuclear transcription factor Y subunit beta |
| NCBI Synonym Full Names: | nuclear transcription factor Y, beta |
| NCBI Official Symbol: | NFYB |
| NCBI Official Synonym Symbols: | HAP3; CBF-A; CBF-B; NF-YB |
| NCBI Protein Information: | nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A |
| UniProt Protein Name: | Nuclear transcription factor Y subunit beta |
| UniProt Synonym Protein Names: | CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB |
| Protein Family: | Nuclear transcription factor |
| UniProt Gene Name: | NFYB |
| UniProt Entry Name: | NFYB_HUMAN |
