Transcription Factor Activity Assay Kits

The Assay Genie Transcription Factor Activity Assay Kits are a premium range ELISA kits used for the detection of transcription factor binding to DNA in nuclear or cell lysates in a simple 96-well format.

Features Applications
  • Simple Measure Transcription Factor DNA-binding in 96-wells simultaneously
  • Rapid Modified ELISA format means rapid results
  • Safer Eliminates the need for radioactivity
  • Sensitive High sensitivity and signal-to-noise ratio.
  • Detect & analyse activate transcription factors in nuclear and cell lysates.
  • Analyse signal transduction pathways
  • Establishment of target candidates in drug development.
  • Screen for inhibitors & activators of transcription factor activity

Transcription Factor Activity Assay Kits

ADA2L FOXO1 (Phospho-Ser256) Oct1
AFX FOXO1 (Phospho-Ser319) Oct2
AML1 (Phospho-Ser303) FOXO1 Oct3
AML1 (Phospho-Ser435) Fra-2 p53 (Acetyl-Lys317)
AML1 GATA1 (Phospho-Ser142) p53 (Phospho-Ser20)
Androgen Receptor (Phospho-Ser650) GATA1 (Phospho-Ser310) p53 (Phospho-Ser315)
Androgen Receptor (Phospho-Tyr363) GATA1 p53 (Phospho-Ser33)
Androgen Receptor GATA3 p53 (Phospho-Ser376)
AP-2 GATA4 (Phospho-Ser105) p53 (Phospho-Ser392)
ATF1 GATA4 (Phospho-Ser262) p53 (Phospho-Thr18)
ATF2 (Phospho-Ser480) GATA4 p53
ATF2 (Phospho-Thr69 or 51) GATA6 p63
ATF2 (Phospho-Thr71 or 53) GR (Phospho-Ser211) p73
ATF2 GR Pax-8
ATF3 GTF2IRD1 POU4F1
ATF5 HBP1 PPAR-alpha
ATF7 HIF-1alpha PPAR-gamma
B-Myb HNF4alpha/gamma Progesterone Receptor
CDX2 HOXA11/D11 Retinoic Acid Receptor alpha (Phospho-Ser77)
CEBPB HOXA1/B1/D1 Retinoic Acid Receptor alpha
CEBPD/E HOXA1 Retinoic Acid Receptor beta
CEBPZ HOXB2 Retinoid X Receptor gamma
c-Jun (Phospho-Ser243) HOXD10 RFX2
c-Jun (Phospho-Ser249) HOXD12 RFX3
c-Jun (Phospho-Ser63) HOXD8 RREB1
c-Jun (Phospho-Ser73) HSF1 (Phospho-Ser303) RUNX3
c-Jun (Phospho-Thr239) HSF1 SIX5
c-Jun (Phospho-Thr91) IKZF2 Smad1 (Phospho-Ser187)
c-Jun (Phospho-Thr93) IKZF4 Smad1 (Phospho-Ser465)
c-Jun IRF2 Smad1
CREB3L2 JunB (Phospho-Ser259) Smad2 (Phospho-Ser250)
CREB (Phospho-Ser121) JunB Smad2
CREB (Phospho-Ser129) JunD (Phospho-Ser255) Smad3 (Phospho-Ser213)
CREB (Phospho-Ser133) KLF10/11 Smad3
CREB KLF7 SMRC2
DLX3 KLF SOX12
DLX5 MAFF SOX6
DP-2 Maf SOX-9 (Phospho-Ser181)
E2F-5 MEF2A (Phospho-Ser408) SP1
EGR1/2 MEF2A (Phospho-Thr312) SP2
ELF1 MEF2A SP3/4
ELF5 MEF2C (Phospho-Ser396) SPI1
Elk1 (Phospho-Ser383) MEF2D (Phospho-Ser444) SPIC
Elk1 (Phospho-Ser389) Meis1 SRF
Elk1 (Phospho-Thr417) MYB (Phospho-Ser12) SRY
Elk1 MYB STAT1 (Phospho-Ser727)
Elk3 (Phospho-Ser357) Myc (Phospho-Ser62) STAT1 (Phospho-Tyr701)
ERF Myc (Phospho-Thr58) STAT1
Estrogen Receptor-alpha (Phospho-Ser118) MYC STAT2
Estrogen Receptor-alpha MYSM1 STAT3
Estrogen Receptor-beta (Phospho-Ser105) NCoR1 STAT5A (Phospho-Ser780)
Estrogen Receptor-beta NCOR2 STAT5A
ETS1 (Phospho-Thr38) NFAT3 STAT5B (Phospho-Ser731)
ETS1 NF-kappaB p100/p52 STAT5B
ETV4 NF-kappaB p100 (Phospho-Ser872) TBX18
ETV6 NF-kappaB p105/p50 TBX1
FKHRL1 (Phospho-Ser253) NF-kappaB p65 (Phospho-Ser276) TBX2
FKHRL1 NF-kappaB p65 (Phospho-Ser311) TBX3
FLI1 NF-kappaB p65 (Phospho-Ser468) TCF7
Fos NF-kappaB p65 (Phospho-Ser529) TCFL5
FOXB2 NF-kappaB p65 TEAD3
FOXC1/2 NFYA Telomeric Repeat Binding Factor 1
FOXD3 NFYB TFE3
FOXH1 NR1I3 Thyroid Hormone Receptor alpha
FOXK1 NR2F6 Thyroid Hormone Receptor beta
FOXL1 NRF1 TTF1
FOXO1A NRL ZEB2

Transcription Factor Activity Assays Overview

The Assay Genie Transcription Factor Activity Assay Kits are a premium range of Enzyme Linked Immunosorbent Assay (ELISA) kits used for the detection of active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie Transcription Factor Activity Assay Kits allow scientists to perform high throughput screening of hundreds of samples in a convenient, efficient, and sensitive manner. This accelerates the journey from research to publication or development of a new drug.


Principle

The Assay Genie Transcription Factor Activity Assay (ELISA) Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA.

Streptavidin is bound to the immunoassay plate and specific biotinylated double stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction.

After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate coated streptavidin.

A HRP-conjugated secondary antibody is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate.

After addition of the substrate, a peroxidase catalysed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Colour development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow.

The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample.

View a Transcription Factor Activity Assay Sample Protocol here.

Figure: Schematic representation of Assay Genie Transcription Factor Activity Assay principle