Phosphorylation ELISA Kits
Measure pathway activation in a 96-well plate. Choose quantitative sandwich ELISA kits for cell and tissue lysates, or cell-based kits that measure phosphorylated and total protein side by side directly in fixed cells.
A sandwich ELISA: a capture antibody binds the target from your cell or tissue lysate, and a phospho-specific detection antibody reports the phosphorylated fraction against a standard curve.
Cells are cultured, treated and fixed in the plate, then probed with phospho-specific, total-protein and GAPDH antibodies — ideal for screening activators and inhibitors.
Grow cells in the 96-well plate and apply your activators or inhibitors.
Fix cells in situ, quench and block — no lysis required.
Add phospho-specific, total-protein and GAPDH antibodies to matched wells.
HRP detection at 450 nm; report phospho-to-total and normalise to GAPDH.
Quantitative sandwich ELISA for lysates.
Tau phosphorylation in neurodegeneration models.
Popular phosphorylation sites grouped by pathway, with the reactivity and format of each kit. Browse the full range in the product listing below.
Reactivity: H = human, M = mouse, R = rat. Quantitative kits use lysates; cell-based and in-cell kits use fixed cells.
A phospho ELISA gives a numeric read-out in a 96-well plate, so you can compare many treatments, doses or time points at once without gels, transfers or densitometry — and with better throughput and reproducibility.
Quantitative kits are sandwich ELISAs that measure the phosphorylated protein in cell or tissue lysates against a standard curve. Cell-based kits measure phospho and total protein directly in fixed cells in the culture plate — no lysate preparation — and report relative levels for comparing treatments.
Each cell-based kit includes antibodies to the phosphorylated target, the total target and GAPDH, so you can report phospho-to-total ratios and normalise to GAPDH. Crystal violet staining can also be used to correct for cell number.
Reactivity varies by kit; many detect human, mouse and rat. The reactivity of each kit is shown in the tables above and on its product page.
The tables show popular sites; many more are in the product listing below. If your target or site isn't available, our PhD-level team can develop a custom phospho ELISA.
