Mitochondrial Activity Assays
Measure the activity of each respiratory chain complex, from complex I through to ATP synthase, in isolated mitochondria, cells and tissue.
NADH-CoQ reductase (complex I) and succinate-linked complex II feed electrons into the chain.
Coenzyme Q-cytochrome c reductase and cytochrome c oxidase carry electrons through to oxygen.
F0F1-ATPase activity reports the final step, where the proton gradient drives ATP production.
Oxidative phosphorylation depends on five protein complexes in the inner mitochondrial membrane. Measuring each complex separately shows where a drug, mutation or disease model disrupts electron transport, which a whole-cell ATP or oxygen read-out cannot resolve.
A mitochondrial extraction kit is available for preparing samples, and upstream TCA cycle and ATP assays complete the picture.
Complex I, II, III, IV and the F0F1-ATPase (ATP synthase).
Sample requirements are set out in each protocol. A mitochondrial extraction kit is available in our extraction kits category.
Yes. Comparing complex activities between treated and control samples is a common way to locate drug-induced mitochondrial dysfunction.