Urea Assays
The urea cycle clears nitrogenous waste as urea. Measure urea and ammonia directly, plus the enzymes, amino acids and metabolites that feed the pathway.
Urea, ammonia and arginase activity, with an arginase inhibitor screening kit.
Glutamate, glutamate dehydrogenase and AST activity.
Fumarate, malate, oxaloacetate, ADP/ATP and NAD/NADH.
The urea cycle converts toxic ammonia, produced by amino acid breakdown, into urea for excretion. It links amino acid metabolism, the TCA cycle (through fumarate and aspartate) and nitrogen balance.
Measuring urea and ammonia alongside the relevant enzymes and intermediates helps dissect nitrogen handling in liver function, metabolism and disease models.
The Urea Assay Kit can use around 5 µL of sample, with a linear range from roughly 0.08 to 100 mg/dL urea in a 96-well format.
No. It is designed to run directly on raw biological samples without ammonia interference and without pretreatment.
The urea cycle connects to the TCA cycle through fumarate and aspartate, so intermediate and transaminase assays place urea production in its metabolic context.
Depending on the kit: serum, plasma, urine, milk, cell and tissue culture, food and environmental samples.