NAD/NADH Assay Kit
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
ApplicationsDirect Assays: NAD+ /NADH concentrations and ratios in cell or tissue extracts.
- Sensitive and accurate. Detection limit of 0.02 uM and linearity up to 1 uM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
NAD/NADH Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL Probe: 750 mL NAD Standard: 0.5 mL|
|Kit Requires:||Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at ex/em = 530/585 nm.|
|Method of Detection:||F530/585nm|
|Detection Limit:||0.02 uM|
|Samples:||Cell, tissue extracts etc.|
|Protocol Length:||10 min|
|Storage:||Store all reagents at -20°C.|
|Shelf Life:||6 months|