NAD/NADH Assay Kit (BA0106)

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SKU:
BA0106

Description

NAD/NADH Assay Kit

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. 

Assay Genies flurormetric NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.

Applications

Direct Assays: NAD+ /NADH concentrations and ratios in cell or tissue extracts.

Key Features

  • Sensitive and accurate. Detection limit of 0.02 uM and linearity up to 1 uM NAD+/NADH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NAD/NADH Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL Probe: 750 mL NAD Standard: 0.5 mL
Kit Requires: Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at ex/em = 530/585 nm.
Method of Detection: F530/585nm
Detection Limit: 0.02 uM
Samples: Cell, tissue extracts etc.
Species: All
Protocol Length: 10 min
Size: 100 tests
Storage: Store all reagents at -20°C.
Shelf Life:  6 months

 

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