Glycolysis & Carbohydrates Assay Kits
Track glycolysis step by step
Glycolysis converts glucose to pyruvate and fuels cellular energy. Our kits quantify the key indicators — glucose and uptake, glycogen and sugars, lactate and pyruvate, and the ATP/NAD(H) energy currency — on a standard plate reader.
Which glycolysis assay do you need?
From glucose and its uptake to lactate, pyruvate and the ATP/NAD(H) energy pool — pick an indicator to filter the range.
All glycolysis & carbohydrate assays
Filter by pathway area or search by analyte or product code.
Why choose these assays?
A complete carbohydrate-metabolism toolkit for the plate reader.
Whole-pathway coverage
Glucose, glycogen, lactate, pyruvate and the ATP/NAD(H) energy currency.
Flexible read-outs
Colorimetric, fluorometric and luminescent formats to match sensitivity and instrument.
Cell & tissue ready
Validated across cells, tissue, serum, plasma and even food and plant samples.
Metabolism-focused
Purpose-built to profile glycolytic flux and carbohydrate handling.
What glycolysis assays measure
Glycolysis breaks glucose down to pyruvate, generating ATP and reducing NAD+ to NADH. Under low oxygen or in many tumours, pyruvate is converted to lactate, making glucose consumption and lactate output classic read-outs of glycolytic activity.
Measuring substrates (glucose, glycogen), products (lactate, pyruvate) and the ATP/NAD(H) energy pool together gives a rounded picture of carbohydrate metabolism.
- Glucose & uptake, glucose-6-phosphate, G6PD
- Glycogen and sugars (sucrose, xylose, lactase)
- Lactate, pyruvate and LDH activity
- Energy nucleotides: ATP, ADP, NAD(P)/NADH
FAQs
How is glycolytic activity measured?
Common read-outs are glucose consumption, lactate production and the ATP/NAD(H) balance. Glucose-uptake and pyruvate assays add further resolution on flux through the pathway.
Why measure lactate for glycolysis?
Under anaerobic conditions and in many cancers, pyruvate is converted to lactate, so lactate output is a widely used indicator of glycolytic (and specifically aerobic-glycolysis) activity.
Which assays use luminescence?
ATP and some carbohydrate assays are available in luminescent formats for high sensitivity; the product name and card indicate the read-out.
What sample types are supported?
Depending on the kit, validated samples include cells, tissue, serum, plasma, urine, whole blood, culture medium, food and plant material.
Profiling glycolytic metabolism?
Our PhD-level technical team can help you build a glucose, lactate and energy-nucleotide panel for your model.


