null

Arrays

Membrane Antibody Arrays

Membrane Antibody Arrays

Like running dozens of ELISAs on a single membrane

Screen 7 to 274 proteins per sample across more than 10 species using standard Western-blotting and chemiluminescent imaging equipment. Ideal for biomarker discovery, pathway profiling and target validation.

Browse all 105 arrays Build my array
Membrane antibody array Duplicate capture-antibody spots · chemiluminescent read-out Signal intensity is proportional to protein abundance ref.
105
Arrays
10
Species
7–274
Targets / array
<5%
Inter-array CV
1 pg/mL
Sensitivity
~24 h
Workflow
Arrays by species

Choose your species

Antibody arrays are available for ten species. Pick a species to filter the full catalogue below.

57 arrays

Human

Cytokines, inflammation, apoptosis, angiogenesis, phosphorylation & more.

View Human arrays → 22 arrays

Mouse

Cytokines, chemokines, inflammation, apoptosis & growth factors.

View Mouse arrays → 7 arrays

Rat

Cytokines, chemokines, growth factors, neuro & immune checkpoint.

View Rat arrays → 21 arrays

Other species

Bovine, Canine, Porcine, Sheep, Dolphin, Horse & Chicken arrays.

View other species →
Why antibody arrays

More proteins, less sample, one experiment

Discovery

Detect 7–274 targets per sample across 10+ species in a single membrane experiment.

Sensitive

Span roughly four orders of magnitude, down to ~1 pg/mL for some proteins.

Reproducible

Inter-array coefficient of variation (CV) as low as 5%.

Easy to use

Pre-spotted capture antibodies give multiplex results in ~24 h on standard Western-blot equipment.

Validation-ready

Confirm hits with PharmaGenie ELISA kits built on the same antibody pairs.

Compatible

Read out on any chemiluminescent imaging system — no specialised instrument needed.

Chemiluminescent lightStreptavidin–HRPDetection antibody(biotinylated)Target proteinCapture antibodyMembrane spot
How it works

A sandwich immunoassay on a membrane

Each array is a nitrocellulose membrane pre-spotted in duplicate with validated capture antibodies. Your sample flows over the membrane and target proteins are trapped by their matched capture antibody.

  • Bound targets are detected with a biotinylated antibody cocktail.
  • Streptavidin-HRP and a chemiluminescent substrate generate light at each spot.
  • Spot intensity is proportional to protein abundance and quantified by densitometry.
  • Duplicate spots and built-in reference points support reliable comparison between samples.
Workflow

Four short steps, results in ~24 hours

The complete membrane array protocol runs on standard Western-blotting equipment.

Incubate sample

Add serum, plasma, lysate or culture supernatant to the blocked membrane.

Detect

Apply the biotinylated detection antibody cocktail.

Develop

Add streptavidin-HRP and the chemiluminescent substrate.

Image & quantify

Capture on any chemiluminescent imager and quantify spot density.

Full catalogue

Browse all 105 antibody arrays

Filter by species and research area, or search by name or catalogue number. Every array links to its product page.

Array ↕ Species ↕ Research area ↕ Targets ↕ Cat. No. ↕
Arrays vs traditional methods

Why screen with an array?

Compared with running one ELISA or Western blot per target, a single membrane array delivers dramatically more information per sample.

Up to 274

More targets

Profile a whole pathway or disease signature in one experiment instead of one protein at a time.

One sample

Less material

Precious or limited samples go further — no need to split across dozens of separate assays.

~24 h

Faster screening

Reach a shortlist of candidate targets in a day, then validate with quantitative ELISA.

Explore more

Related tools & resources

Browse by research areaFilter all 105 arrays by pathway or disease area PharmaGenie ELISA KitsValidate array hits with matched antibody pairs GeniePlex Multiplex AssaysQuantitative bead-based multiplex by flow cytometry Talk to a scientistFree advice on choosing or customising an array
FAQ

Frequently asked questions

What is a membrane antibody array?
A membrane antibody array is a nitrocellulose membrane pre-spotted in duplicate with capture antibodies against a defined panel of proteins. Your sample is incubated on the membrane and bound targets are detected with a biotinylated antibody cocktail and chemiluminescence, letting you profile many proteins from a single sample at once.
How many proteins can I detect on one array?
Assay Genie membrane antibody arrays detect between 7 and 274 proteins per sample depending on the array chosen, across more than 10 species.
Which sample types are compatible?
Arrays work with serum, plasma, cell and tissue lysates, cell-culture supernatants and other biological fluids. No sample enrichment or specialist labelling is required.
Is the data quantitative?
Membrane arrays give relative (semi-quantitative) profiling that is ideal for screening and discovery. Once you identify targets of interest, you can quantify them precisely using PharmaGenie ELISA kits built on the same antibody pairs.
What equipment do I need?
Standard Western-blotting incubation equipment and any chemiluminescent imaging system. No dedicated array reader or flow cytometer is required.
Which species are available?
Human, Mouse, Rat, Bovine, Canine, Porcine, Sheep, Dolphin, Horse (Equine) and Chicken (Gallus) arrays are available.
How do I validate array hits?
Confirm and quantify your top hits with PharmaGenie ELISA kits, which use the same validated antibody pairs as the membrane arrays for a seamless discovery-to-validation workflow.
How long does the assay take?
A typical membrane antibody array workflow is completed in around 24 hours across a series of short incubations.

Not sure which array fits your study?

Tell us your species, sample type and the targets you care about, and our scientific team will recommend the right membrane array — free of charge.

Talk to our team
105 Antibody arrays 10 Species covered <5% Inter-array CV 1 pg/mL Sensitivity PhD Level support