Description
system_update_altDatasheet
Human Cytokine Array (51 targets) (SARB0044)
The Human Cytokine Array (51 Targets) is a powerful tool for researchers studying cytokine expression levels in human samples. This array allows for the simultaneous detection and quantification of 51 different cytokines, providing a comprehensive overview of the immune response in various cell types.With its high sensitivity and specificity, this array is ideal for studying a wide range of conditions, including inflammation, autoimmune diseases, and cancer. By measuring the levels of multiple cytokines in a single sample, researchers can gain valuable insights into the complex interactions that drive these diseases.
The Human Cytokine Array (51 Targets) is easy to use and provides reliable and reproducible results. Its high-throughput format makes it a cost-effective solution for screening large numbers of samples. Whether you are studying basic immune function or searching for potential biomarkers for disease, this array offers a convenient and efficient way to analyze cytokine expression profiles in human samples.
| Product Sku: | SARB0044 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 51 |
| Gene Symbols: | ADAM17, AGER, BCAM, CD40, CFD, CGA, EDA2R, FCGR2B, FST, FSTL3|FTH1, FURIN, GDF15, GH1, HAVCR1, IFNL1, IFNL2, IL10RA, IL22, IL31, INS, KLK3, LGALS7, LHB, LYVE1, MICA, MICB, MMP10, MMP2, MMP7, MMP8, NCAM1, NID1, NRCAM, NRG1, PF4, PI3, PRSS27, RETN, SAA1, SCARB2, SERPINE1, SIGLEC9, SPP1, THRB, TNFRSF10B, TNFRSF11A, TNFRSF8, TREM1, TSLP, VCAM1, VEGFC |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adipsin (Complement Factor D)
| BCAM
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
| Fc gamma RIIB/C (CD32B/C)
|
Ferritin
| FLRG
| Follistatin
| Furin
| Galectin-7
|
GDF-15
| Growth Hormone
| IL-10 R alpha
| IL-22
| IL-28A (IFN-lambda 2)
|
IL-29 (IFN-lambda 1)
| IL-31
| Insulin
| Luteinizing hormone
| LIMPII
|
LYVE-1
| Marapsin
| MICA
| MICB
| MMP-2
|
MMP-7
| MMP-8
| MMP-10
| NCAM-1 (CD56)
| Nidogen-1
|
NrCAM
| NRG1-beta 1 (HRG1-beta 1)
| Osteopontin (SPP1)
| PAI-1
| Platelet factor 4 (CXCL4)
|
PSA-total
| RAGE
| RANK (TNFRSF11A)
| Resistin
| SAA (Serum Amyloid A)
|
Siglec-9
| TACE
| TIM-1 (KIM-1)
| TRAIL R2 (TNFRSF10B/DR5)
| Trappin-2
|
TREM-1
| TSH
| TSLP
| VCAM-1 (CD106)
| VEGF-C
|
XEDAR
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C9 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
