Human Cytokine Array (51 targets) (SARB0044)

SKU:
SARB0044
€904 - €1,868

Description

Human Cytokine Array Product Information

Product Sku:SARB0044
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:51
Gene Symbols:ADAM17, AGER, BCAM, CD40, CFD, CGA, EDA2R, FCGR2B, FST, FSTL3|FTH1, FURIN, GDF15, GH1, HAVCR1, IFNL1, IFNL2, IL10RA, IL22, IL31, INS, KLK3, LGALS7, LHB, LYVE1, MICA, MICB, MMP10, MMP2, MMP7, MMP8, NCAM1, NID1, NRCAM, NRG1, PF4, PI3, PRSS27, RETN, SAA1, SCARB2, SERPINE1, SIGLEC9, SPP1, THRB, TNFRSF10B, TNFRSF11A, TNFRSF8, TREM1, TSLP, VCAM1, VEGFC
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Adipsin (Complement Factor D)BCAMCD30 (TNFRSF8)CD40 (TNFRSF5)Fc gamma RIIB/C (CD32B/C)
FerritinFLRGFollistatinFurinGalectin-7
GDF-15Growth HormoneIL-10 R alphaIL-22IL-28A (IFN-lambda 2)
IL-29 (IFN-lambda 1)IL-31InsulinLuteinizing hormoneLIMPII
LYVE-1MarapsinMICAMICBMMP-2
MMP-7MMP-8MMP-10NCAM-1 (CD56)Nidogen-1
NrCAMNRG1-beta 1 (HRG1-beta 1)Osteopontin (SPP1)PAI-1Platelet factor 4 (CXCL4)
PSA-totalRAGERANK (TNFRSF11A)ResistinSAA (Serum Amyloid A)
Siglec-9TACETIM-1 (KIM-1)TRAIL R2 (TNFRSF10B/DR5)Trappin-2
TREM-1TSHTSHBTSLPVCAM-1 (CD106)
VEGF-CXEDAR   

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C9 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Adipsin (Complement Factor D)
Target:
BCAM
Target:
CD30 (TNFRSF8)
Target:
CD40 (TNFRSF5)
Target:
Fc gamma RIIB/C (CD32B/C)
Target:
Ferritin
Target:
FLRG
Target:
Follistatin
Target:
Furin
Target:
Galectin-7
Target:
GDF-15
Target:
Growth Hormone
Target:
IL-10 R alpha
Target:
IL-22
Target:
IL-28A (IFN-lambda 2)
Target:
IL-29 (IFN-lambda 1)
Target:
IL-31
Target:
Insulin
Target:
Luteinizing hormone
Target:
LIMPII
Target:
LYVE-1
Target:
Marapsin
Target:
MICA
Target:
MICB
Target:
MMP-2
Target:
MMP-7
Target:
MMP-8
Target:
MMP-10
Target:
NCAM-1 (CD56)
Target:
Nidogen-1
Target:
NrCAM
Target:
NRG1-beta 1 (HRG1-beta 1)
Target:
Osteopontin (SPP1)
Target:
PAI-1
Target:
Platelet factor 4 (CXCL4)
Target:
PSA-total
Target:
RAGE
Target:
RANK (TNFRSF11A)
Target:
Resistin
Target:
SAA (Serum Amyloid A)
Target:
Siglec-9
Target:
TACE
Target:
TIM-1 (KIM-1)
Target:
TRAIL R2 (TNFRSF10B/DR5)
Reactivity:
Human
Application:
Multiplex Array
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