Human Cytokine Array (80 targets) (SARB0040)

SKU:
SARB0040
€644 - €1,297

Description

Human Cytokine Array Product Information

Product Sku:SARB0040
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:80
Gene Symbols:ANG, BDNF, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20|CCL22, CCL23, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CSF1, CSF2, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL10, IL12A, IL13, IL15, IL16, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LIF, LTA, MIF, NTF3, NTF4, OSM, PDGFB, PGF, PPBP, SPP1, TGFB1, TGFB2, TGFB3, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFSF14, TPO, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

AngiogeninBDNFBLC (CXCL13)CK beta 8-1 (CCL23)EGF
ENA-78 (CXCL5)Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)Eotaxin-3 (CCL26)FGF-4
FGF-6FGF-7 (KGF)FGF-9Flt-3 LigandFractalkine (CX3CL1)
GCP-2 (CXCL6)GCSFGDNFGM-CSFGRO alpha (CXCL1)
GRO alpha/beta/gammaHGFI-309 (TCA-3/CCL1)IFN-gammaIGF-1
IGFBP-1IGFBP-2IGFBP-3IGFBP-4IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)IL-2IL-3IL-4IL-5
IL-6IL-7IL-8 (CXCL8)IL-10IL-12 p40/p70
IL-13IL-15IL-16IP-10 (CXCL10)Leptin
LIFLIGHT (TNFSF14)MCP-1 (CCL2)MCP-2 (CCL8)MCP-3 (MARC/CCL7)
MCP-4 (CCL13)M-CSFMDC (CCL22)MIFMIG (CXCL9)
MIP-1 beta (CCL4)6351MIP-1 delta (CCL15)MIP-3 alpha (CCL20)NAP-2 (PPBP/CXCL7)
NT-3NT-4Oncostatin MOsteopontin (SPP1)Osteoprotegerin (TNFRSF11B)
PARC (CCL18)PDGF-BBPLGFRANTES (CCL5)SCF
SDF-1 alpha (CXCL12 alpha)TARC (CCL17)TGF beta 1TGF beta 2TGF beta 3
Thrombopoietin (TPO)TIMP-1TIMP-2TNF alphaTNF beta (TNFSF1B)
VEGF-A    

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Angiogenin
Target:
BDNF
Target:
BLC (CXCL13)
Target:
CK beta 8-1 (CCL23)
Target:
EGF
Target:
ENA-78 (CXCL5)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Eotaxin-3 (CCL26)
Target:
FGF-4
Target:
FGF-6
Target:
FGF-7 (KGF)
Target:
FGF-9
Target:
Flt-3 Ligand
Target:
Fractalkine (CX3CL1)
Target:
GCP-2 (CXCL6)
Target:
GCSF
Target:
GDNF
Target:
GM-CSF
Target:
GRO alpha (CXCL1)
Target:
GRO alpha/beta/gamma
Target:
HGF
Target:
I-309 (TCA-3/CCL1)
Target:
IFN-gamma
Target:
IGF-1
Target:
IGFBP-1
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-4
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-2
Target:
IL-3
Target:
IL-4
Target:
IL-5
Target:
IL-6
Target:
IL-7
Target:
IL-8 (CXCL8)
Target:
IL-10
Target:
IL-12 p40/p70
Target:
IL-13
Target:
IL-15
Target:
IL-16
Target:
IP-10 (CXCL10)
Reactivity:
Human
Application:
Multiplex Array
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