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Human Cytokine Array (23 targets) (SARB0034)

SKU:
SARB0034
Product Type:
Protein Array
Analytes:
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha (CXCL1)
IL-1 alpha (IL-1 F1)
IL-2
IL-3
IL-5
IL-6
IL-7
IL-8 (CXCL8)
IL-10
IL-13
IL-15
IFN-gamma
MCP-1 (CCL2)
MCP-2 (CCL8)
MCP-3 (MARC/CCL7)
MIG (CXCL9)
RANTES (CCL5)
TGF beta 1
TNF alpha
TNF beta (TNFSF1B)
Reactivity:
Human
Applications:
Multiplex Array
€669 - €909
Frequently bought together:

Description

system_update_altDatasheet
Product Sku:SARB0034
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:23
Gene Symbols:CCL2, CCL5, CCL7, CCL8, CSF2, CSF3, CXCL1, CXCL8, CXCL9, IFNG|IL10, IL13, IL15, IL1A, IL2, IL3, IL5, IL6, IL7, LTA, TGFB1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 1440
  • Accession #: P09919
GM-CSF
  • Gene Symbol: CSF2 GMCSF
  • Gene ID: 1437
  • Accession #: P04141
GRO alpha/beta/gamma
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1
  • Gene ID: 2919
  • Accession #: P09341
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A IL1F1
  • Gene ID: 3552
  • Accession #: P01583
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6 IFNB2
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #: P10145
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-13
  • Gene Symbol: IL13 NC30
  • Gene ID: 3596
  • Accession #: P35225
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
MCP-1 (CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8 MCP2 SCYA10 SCYA8
  • Gene ID: 6355
  • Accession #: P80075
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7 MCP3 SCYA6 SCYA7
  • Gene ID: 6354
  • Accession #: P80098
MIG (CXCL9)
  • Gene Symbol: CXCL9 CMK MIG SCYB9
  • Gene ID: 4283
  • Accession #: Q07325
RANTES (CCL5)
  • Gene Symbol: CCL5 D17S136E SCYA5
  • Gene ID: 6352
  • Accession #: P13501
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA TNFB TNFSF1
  • Gene ID: 4049
  • Accession #: P01374
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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