Human Cytokine Array (23 targets) (SARB0034)

SKU:
SARB0034
€631 - €862

Description

Human Cytokine Array Product Information

Product Sku:SARB0034
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:23
Gene Symbols:CCL2, CCL5, CCL7, CCL8, CSF2, CSF3, CXCL1, CXCL8, CXCL9, IFNG|IL10, IL13, IL15, IL1A, IL2, IL3, IL5, IL6, IL7, LTA, TGFB1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 1440
  • Accession #: P09919
GM-CSF
  • Gene Symbol: CSF2 GMCSF
  • Gene ID: 1437
  • Accession #: P04141
GRO alpha/beta/gamma
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1
  • Gene ID: 2919
  • Accession #: P09341
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A IL1F1
  • Gene ID: 3552
  • Accession #: P01583
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6 IFNB2
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #: P10145
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-13
  • Gene Symbol: IL13 NC30
  • Gene ID: 3596
  • Accession #: P35225
  

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
GCSF
Target:
GM-CSF
Target:
GRO alpha/beta/gamma
Target:
GRO alpha (CXCL1)
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-2
Target:
IL-3
Target:
IL-5
Target:
IL-6
Target:
IL-7
Target:
IL-8 (CXCL8)
Target:
IL-10
Target:
IL-13
Target:
IL-15
Target:
IFN-gamma
Target:
MCP-1 (CCL2)
Target:
MCP-2 (CCL8)
Target:
MCP-3 (MARC/CCL7)
Target:
MIG (CXCL9)
Target:
RANTES (CCL5)
Target:
TGF beta 1
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Reactivity:
Human
Application:
Multiplex Array
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