Human Cytokine Array (120 targets) (SARB0031)

SKU:
SARB0031
€904 - €1,868

Description

Human Cytokine Array Product Information

Product Sku:SARB0031
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:120
Gene Symbols:ADIPOQ, AGRP, ANG, ANGPT2, AREG, AXL, BDNF, BMP4, BMP6, BTC|CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CNTF, CSF1, CSF2, CSF3, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, EGFR, FAS, FGF2, FGF4, FGF6, FGF7, FGF9, FIGF, FLT3LG, GDNF, HGF, ICAM1, ICAM3, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, IL10, IL11, IL12A, IL12B, IL13, IL15, IL16, IL17A, IL1A, IL1B, IL1R1, IL1RL1, IL1RN, IL2, IL2RA, IL3, IL4, IL5, IL6, IL6R, IL6ST, IL7, KITLG, LEP, LTA, MIF, MST1, NGF, NTF3, NTF4, OSM, PDGFB, PGF, PLAUR, PPBP, TGFB1, TGFB3, THPO, TIMP1, TIMP2, TNF, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFSF14, TNFSF18, TPO, TYRO3, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Adiponectin (ACRP30)AgRPAmphiregulinAngiogeninAngiopoietin-2
AxlBDNFbFGFBLC (CXCL13)BMP-4
BMP-6beta-NGFBetacellulin (BTC)CCL28 (MEC)CK beta 8-1 (CCL23)
CNTFCTACK (CCL27)DtkEGFEGFR
ENA-78 (CXCL5)Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)Eotaxin-3 (CCL26)Fas (TNFRSF6/Apo-1)
FGF-4FGF-6FGF-7 (KGF)FGF-9Flt-3 Ligand
Fractalkine (CX3CL1)GCP-2 (CXCL6)GCSFGDNFGITR (TNFRSF18)
GITR Ligand (TNFSF18)GM-CSFGRO alpha/beta/gammaGRO alpha (CXCL1)HCC-4 (CCL16)
HGFI-309 (TCA-3/CCL1)ICAM-1 (CD54)ICAM-3 (CD50)IFN-gamma
IGFBP-1IGFBP-2IGFBP-3IGFBP-4IGFBP-6
IGF-1IGF-1 RIL-1 R4 (ST2)IL-1 R1IL-10
IL-11IL-12 p40IL-12 p70IL-13IL-15
IL-16IL-17AIL-1 alpha (IL-1 F1)IL-1 beta (IL-1 F2)IL-1 ra (IL-1 F3)
IL-2IL-2 R alphaIL-3IL-4IL-5
IL-6IL-6 RIL-7IL-8 (CXCL8)I-TAC (CXCL11)
LeptinLight (TNFSF14)Lymphotactin (XCL1)MCP-1 (CCL2)MCP-2 (CCL8)
MCP-3 (MARC/CCL7)MCP-4 (CCL13)M-CSFMDC (CCL22)MIF
MIG (CXCL9)MIP-1 alpha (CCL3)MIP-1 beta (CCL4)MIP-1 delta (CCL15)MIP-3 alpha (CCL20)
MIP-3 beta (CCL19)MSP alpha/betaNAP-2 (PPBP/CXCL7)NT-3NT-4
Oncostatin MOsteoprotegerin (TNFRSF11B)PARC (CCL18)PDGF-BBPLGF
RANTES (CCL5)SCFSDF-1 alpha (CXCL12 alpha)gp130TNF RII (TNFRSF1B)
TNF RI (TNFRSF1A)TARC (CCL17)TECK (CCL25)TGF beta 3TGF beta 1
Thrombopoietin (TPO)TIMP-1TIMP-2TNF alphaTNF beta (TNFSF1B)
TRAIL R3 (TNFRSF10C)TRAIL R4 (TNFRSF10D)uPARVEGF-AVEGF-D

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Adiponectin (ACRP30)
Target:
AgRP
Target:
Amphiregulin
Target:
Angiogenin
Target:
Angiopoietin-2
Target:
Axl
Target:
BDNF
Target:
bFGF
Target:
BLC (CXCL13)
Target:
BMP-4
Target:
BMP-6
Target:
beta-NGF
Target:
Betacellulin (BTC)
Target:
CCL28 (MEC)
Target:
CK beta 8-1 (CCL23)
Target:
CNTF
Target:
CTACK (CCL27)
Target:
Dtk
Target:
EGF
Target:
EGFR
Target:
ENA-78 (CXCL5)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Eotaxin-3 (CCL26)
Target:
Fas (TNFRSF6/Apo-1)
Target:
FGF-4
Target:
FGF-6
Target:
FGF-7 (KGF)
Target:
FGF-9
Target:
Flt-3 Ligand
Target:
Fractalkine (CX3CL1)
Target:
GCP-2 (CXCL6)
Target:
GCSF
Target:
GDNF
Target:
GITR (TNFRSF18)
Target:
GITR Ligand (TNFSF18)
Target:
GM-CSF
Target:
GRO alpha/beta/gamma
Target:
GRO alpha (CXCL1)
Target:
HCC-4 (CCL16)
Target:
HGF
Target:
I-309 (TCA-3/CCL1)
Target:
ICAM-1 (CD54)
Target:
ICAM-3 (CD50)
Reactivity:
Human
Application:
Multiplex Array
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