Description
Human Cytokine Array (36 targets) (SARB0039)
The Human Cytokine Array (36 Targets) is a powerful tool for researchers studying cytokine expression profiles in human samples. This array allows for the simultaneous detection of 36 different cytokines, growth factors, and other signaling molecules in a single experiment, providing a comprehensive overview of immune responses and signaling pathways.Each array is carefully designed to include key cytokines involved in inflammation, immune response, cell proliferation, and differentiation. With a focus on cytokines such as IL-6, TNF-alpha, and IFN-gamma, this array is ideal for studying the complex interplay of cytokine networks in various biological processes.The Human Cytokine Array is easy to use, requiring only a small amount of sample for analysis.
It is compatible with a variety of sample types, including serum, plasma, cell culture supernatants, and tissue lysates. Researchers can quickly and efficiently screen multiple samples for cytokine expression levels, making it a valuable tool for drug discovery, biomarker identification, and basic research in immunology and inflammation.Overall, the Human Cytokine Array (36 Targets) provides a reliable and high-throughput solution for studying cytokine profiles in human samples, facilitating the discovery of novel biomarkers and potential therapeutic targets in various diseases and conditions.
| Product Sku: | SARB0039 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 36 |
| Gene Symbols: | BDNF, CCL11, CCL13, CCL18, CCL20, CCL23, CCL24, CCL26, CX3CL1, CXCL10|CXCL13, CXCL6, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL16, LIF, MIF, NTF3, NTF4, PGF, PPBP, TGFB2, TGFB3, TIMP1, TIMP2, TNFRSF11B, TNFSF14 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-4
| FGF-6
| FGF-7 (KGF)
| FGF-9
|
Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
| GDNF
| HGF
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-16
|
LIF
| LIGHT (TNFSF14)
| MCP-4 (CCL13)
| MIF
| NAP-2 (PPBP/CXCL7)
|
NT-3
| NT-4
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
| PLGF
|
TGF beta 2
| TGF beta 3
| TIMP-1
| TIMP-2
| MIP-3 alpha (CCL20)
|
IP-10 (CXCL10)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C4 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
