Human Cytokine Array (36 targets) (SARB0039)

SKU:
SARB0039
€577 - €1,149

Description

Human Cytokine Array Product Information

Product Sku:SARB0039
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:36
Gene Symbols:BDNF, CCL11, CCL13, CCL18, CCL20, CCL23, CCL24, CCL26, CX3CL1, CXCL10|CXCL13, CXCL6, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL16, LIF, MIF, NTF3, NTF4, PGF, PPBP, TGFB2, TGFB3, TIMP1, TIMP2, TNFRSF11B, TNFSF14
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

BDNFBLC (CXCL13)CK beta 8-1 (CCL23)Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)
Eotaxin-3 (CCL26)FGF-4FGF-6FGF-7 (KGF)FGF-9
Flt-3 LigandFractalkine (CX3CL1)GCP-2 (CXCL6)GDNFHGF
IGFBP-1IGFBP-2IGFBP-3IGFBP-4IL-16
LIFLIGHT (TNFSF14)MCP-4 (CCL13)MIFNAP-2 (PPBP/CXCL7)
NT-3NT-4Osteoprotegerin (TNFRSF11B)PARC (CCL18)PLGF
TGF beta 2TGF beta 3TIMP-1TIMP-2MIP-3 alpha (CCL20)
IP-10 (CXCL10)    

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
BDNF
Target:
BLC (CXCL13)
Target:
CK beta 8-1 (CCL23)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Eotaxin-3 (CCL26)
Target:
FGF-4
Target:
FGF-6
Target:
FGF-7 (KGF)
Target:
FGF-9
Target:
Flt-3 Ligand
Target:
Fractalkine (CX3CL1)
Target:
GCP-2 (CXCL6)
Target:
GDNF
Target:
HGF
Target:
IGFBP-1
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-4
Target:
IL-16
Target:
LIF
Target:
LIGHT (TNFSF14)
Target:
MCP-4 (CCL13)
Target:
MIF
Target:
NAP-2 (PPBP/CXCL7)
Target:
NT-3
Target:
NT-4
Target:
Osteoprotegerin (TNFRSF11B)
Target:
PARC (CCL18)
Target:
PLGF
Target:
TGF beta 2
Target:
TGF beta 3
Target:
TIMP-1
Target:
TIMP-2
Target:
MIP-3 alpha (CCL20)
Target:
IP-10 (CXCL10)
Reactivity:
Human
Application:
Multiplex Array
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