Cell Viability Assays
Quantify metabolically active cells in 2D cultures and 3D organoids with add-and-read assays for the plate reader.
Add-and-read reagents report the fraction of metabolically active cells in each well, read by absorbance or fluorescence.
A dedicated assay for organoids and other 3D culture models.
MTT and LDH kits are in cytotoxicity, CCK-8/WST-8 in proliferation, and ATP luminescence (CellQuant-Lux) in luciferase assays.
Viability assays report the fraction of metabolically active cells; cytotoxicity assays report membrane damage and cell death; proliferation assays report the rate of cell division. Choosing the right read-out, or combining them, gives an accurate picture of how cells respond to a treatment.
It measures the proportion of live, metabolically active cells in a sample, usually through a metabolic indicator read by absorbance, fluorescence or luminescence.
Yes. The 3D Organoid Cell Viability Assay is designed for 3D models; for ATP-based viability in spheroids see CellQuant-Lux 3D in luciferase assays.
Yes. Microplate formats with add-and-read workflows suit drug screening and toxicity testing.