TSG101
TSG101 (tumour susceptibility gene 101, also known as VPS23 and TSG10) is a component of the ESCRT-I complex and a regulator of vesicular trafficking. It binds ubiquitinated cargo and sorts it into multivesicular bodies. Browse Assay Genie's current range of TSG101 research reagents below, or read on for the biology behind the target.
TSG101 is a component of the ESCRT-I complex, which regulates vesicular trafficking. It binds ubiquitinated cargo proteins and is required for sorting endocytic ubiquitinated cargo into multivesicular bodies (MVBs). It also mediates the association between the ESCRT-0 and ESCRT-I complexes. Beyond endosomal sorting, TSG101 is required for completion of cytokinesis, a function that depends on CEP55, and for the exosomal release of SDCBP, CD63 and syndecan. It may also contribute to the extracellular release of microvesicles that differ from exosomes.
TSG101 is also involved in the budding of many viruses. It interacts with viral proteins carrying the late-budding motif P-[ST]-A-P, and this interaction is essential for viral particle budding of numerous retroviruses. In cells, the protein is found in the cytoplasm, on early and late endosome membranes, at the centrosome, at the midbody ring and in the nucleus.
The TSG101 gene product belongs to the ubiquitin-conjugating enzyme family, UEV subfamily, a group of apparently inactive homologues of ubiquitin-conjugating enzymes. Its coiled-coil domain interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. TSG101 may be involved in cell growth and differentiation and acts as a negative growth regulator. In vitro, its steady-state expression appears important for genomic stability and cell cycle regulation. Mutations and alternative splicing in the gene occur at high frequency in breast cancer, suggesting that defects arise during tumorigenesis and/or progression. The gene was named for this tumour-suppressor-like behaviour and was previously called tumour susceptibility gene 10; it has orthologues in mouse and rat (Tsg101).
TSG101 binds ubiquitinated cargo and directs it into multivesicular bodies, making it a core subject in studies of ESCRT-dependent trafficking.
TSG101 is required for exosomal release of SDCBP, CD63 and syndecan, and is relevant to studies of extracellular vesicle release.
Through P-[ST]-A-P late-budding motifs, TSG101 is essential for budding of numerous retroviruses, supporting research on virus release.
TSG101 is needed for cytokinesis, regulates growth and genomic stability, and is altered in breast cancer, linking it to tumorigenesis studies.
TSG101 is part of the ESCRT-I complex. It binds ubiquitinated cargo and sorts it into multivesicular bodies, links ESCRT-0 to ESCRT-I, and is required for cytokinesis with CEP55. It also supports exosome release and the budding of many viruses.
TSG101 is also known as VPS23 and TSG10. Earlier names were tumour susceptibility gene 10 and tumour susceptibility gene 101. UniProt lists the protein as tumour susceptibility gene 101 protein, and HGNC places it in the ESCRT-I gene group.
NCBI Gene reports that mutations and alternative splicing of TSG101 occur at high frequency in breast cancer. Its retroviral budding role is also well documented. Orthologues exist in mouse and rat, both named Tsg101; check the datasheet of any product for its validated reactivity.
Other ESCRT components, exosome markers and cytokinesis-related targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q99816; product specifications from Assay Genie datasheets.