P4HA1
P4HA1 (prolyl 4-hydroxylase subunit alpha 1, also known as C-P4Halpha(I) or P4HA) is an alpha subunit of prolyl 4-hydroxylase, a key enzyme in collagen synthesis. It catalyses the formation of 4-hydroxyproline in collagens and other proteins. Browse Assay Genie's current range of P4HA1 research reagents below, or read on for the biology behind the target.
P4HA1 catalyses the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This hydroxylation is essential to the proper three-dimensional folding of newly synthesised procollagen chains. The P4HA1 protein provides the major part of the catalytic site of the active enzyme, which is classed as EC 1.14.11.2.
The active prolyl 4-hydroxylase enzyme is composed of two identical alpha subunits and two beta subunits. The P4HA1 gene encodes one of several different types of alpha subunit, and the protein belongs to the P4HA family. It is located in the lumen of the endoplasmic reticulum. Alternatively spliced transcript variants encoding different isoforms have been described for the P4HA1 gene.
HGNC places P4HA1 in the group of iron (II) and 2-oxoglutarate dependent oxygenases, and in the group of prolyl 4-hydroxylase subunits. Earlier names for the gene include procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, and prolyl 4-hydroxylase, alpha polypeptide I. Mouse and rat orthologs are both designated P4ha1.
P4HA1 forms part of prolyl 4-hydroxylase, a key enzyme in collagen synthesis, making it a focus for studies of procollagen processing.
Formation of 4-hydroxyproline by P4HA1 is essential to the proper three-dimensional folding of newly synthesised procollagen chains.
P4HA1 hydroxylates proline in -Xaa-Pro-Gly- sequences in collagens and other proteins, providing a model for proline hydroxylation.
As an iron (II) and 2-oxoglutarate dependent oxygenase, P4HA1 is relevant to research on this enzyme group and its catalytic mechanism.
P4HA1 catalyses the formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. It provides the major part of the catalytic site of prolyl 4-hydroxylase, and this hydroxylation is essential for correct folding of newly synthesised procollagen chains.
P4HA1 is also known as C-P4Halpha(I) and P4HA. Older names include prolyl 4-hydroxylase, alpha polypeptide I. Alternatively spliced transcript variants encoding different isoforms have been described, so check which isoform is relevant to your work.
No. The active enzyme comprises two identical alpha subunits and two beta subunits, and P4HA1 is one of several alpha subunit types. Mouse and rat orthologs, both named P4ha1, exist; check the datasheet of any product for its validated reactivity.
Other prolyl 4-hydroxylase subunits, collagen-modifying enzymes and collagen targets from the same biological pathway.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P13674; product specifications from Assay Genie datasheets.