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Anti-P4HA1 Antibody (CAB10538)

SKU:
CAB10538
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 2 Anti-P4HA1 Antibody CAB10538
  • Cell Biology Antibodies 2 Anti-P4HA1 Antibody CAB10538
€139 - €419
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Description

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Antibody Name:Anti-P4HA1 Antibody
Antibody SKU:CAB10538
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 235-534 of human P4HA1 (NP_001017962.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:U-87MG, LO2, A-431, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 235-534 of human P4HA1 (NP_001017962.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DPEH QRAN GNLK YFEY IMAK EKDV NKSA SDDQ SDQK TTPK KKGV AVDY LPER QKYE MLCR GEGI KMTP RRQK KLFC RYHD GNRN PKFI LAPA KQED EWDK PRII RFHD IISD AEIE IVKD LAKP RLRR ATIS NPIT GDLE TVHY RISK SAWL SGYE NPVV SRIN MRIQ DLTG LDVS TAEE LQVA NYGV GGQY EPHF DFAR KDEP DAFK ELGT GNRI ATWL FYMS DVSA GGAT VFPE VGAS VWPK KGTA VFWY NLFA SGEG DYST RHAA CPVL VGNK WVSN KWLH ERGQ EFRR PCTL SELE
Gene ID:5033
Uniprot:P13674
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:58kDa/60kDa/61kDa
Observed MW:61kDa
Synonyms:P4HA1, P4HA
Background:This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.11.2; Endoplasmic reticulum; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding

Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline

NCBI Summary:This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P13674
NCBI GenInfo Identifier:2507090
NCBI Gene ID:5033
NCBI Accession:P13674.2
UniProt Secondary Accession:P13674,Q15082, Q15083, Q5VSQ5, C9JL12,
UniProt Related Accession:P13674
Molecular Weight:534
NCBI Full Name:Prolyl 4-hydroxylase subunit alpha-1
NCBI Synonym Full Names:prolyl 4-hydroxylase, alpha polypeptide I
NCBI Official Symbol:P4HA1  
NCBI Official Synonym Symbols:P4HA  
NCBI Protein Information:prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I
UniProt Protein Name:Prolyl 4-hydroxylase subunit alpha-1
UniProt Synonym Protein Names:Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
Protein Family:Prolyl 4-hydroxylase
UniProt Gene Name:P4HA1  
UniProt Entry Name:P4HA1_HUMAN
Anti-P4HA1 Antibody (CAB10538)Western blot analysis of extracts of various cell lines, using P4HA1 antibody (CAB10538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 8s.
Anti-P4HA1 Antibody (CAB10538)Immunofluorescence analysis of L929 cells using P4HA1 Rabbit pAb (CAB10538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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