SENP5
SENP5 (SUMO specific peptidase 5, also known as sentrin-specific protease 5 or MGC27076) is a nucleolar cysteine protease of the peptidase C48 family. It processes SUMO precursors and removes SUMO2 and SUMO3 from modified proteins, and it is required for cell division. Browse Assay Genie's current range of SENP5 research reagents below, or read on for the biology behind the target.
SENP5 is a protease that catalyses two essential functions in the SUMO pathway. First, it processes full-length SUMO3 to its mature form. Second, it deconjugates SUMO2 and SUMO3 from targeted proteins. Its proteolytic activity against full-length SUMO1 or SUMO1 conjugates is weak, which gives SENP5 a preference for SUMO2 and SUMO3 over SUMO1. The protein is required for cell division.
Reversible modification of proteins by small ubiquitin-like SUMO proteins is needed for numerous biological processes. SUMO-specific proteases such as SENP5 have two roles. They carry out the initial processing of SUMO precursors, which generates the C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity, removing SUMO from high molecular mass SUMO conjugates. SENP5 therefore acts at both ends of the SUMO cycle: preparing SUMO for conjugation and reversing the modification.
The SENP5 protein belongs to the peptidase C48 family and is assigned EC 3.4.22.-. UniProt places it in the nucleus, in the nucleolus. The SENP5 gene is classed by HGNC in the SUMO specific peptidases group, alongside SENP1, SENP2 and SENP7. Earlier gene names were SUMO1/sentrin specific protease 5 and SUMO1/sentrin specific peptidase 5, and MGC27076 is a previous symbol. The mouse ortholog is Senp5.
SENP5 both matures SUMO3 and removes SUMO2 and SUMO3 from substrates, so it is a key enzyme for studying the SUMO conjugation cycle.
SENP5 is required for cell division, making it relevant to work on how SUMO processing and deconjugation support cell cycle progression.
SENP5 localises to the nucleolus, so it is a useful candidate for investigating SUMO regulation of nucleolar proteins.
SENP5 acts strongly on SUMO2 and SUMO3 but only weakly on SUMO1, offering a model for how SENP proteases discriminate between SUMO paralogues.
SENP5 is a SUMO-specific protease. It processes full-length SUMO3 to its mature form and deconjugates SUMO2 and SUMO3 from target proteins. Its activity toward SUMO1 or SUMO1 conjugates is weak. SENP5 is also required for cell division.
SENP5 stands for SUMO specific peptidase 5 and is also called sentrin-specific protease 5. Previous gene names were SUMO1/sentrin specific protease 5 and SUMO1/sentrin specific peptidase 5, and MGC27076 is a previous symbol. HGNC groups it with the SUMO specific peptidases.
A mouse ortholog, Senp5, is recorded for the human SENP5 gene. The protein belongs to the peptidase C48 family, which includes other SUMO-specific proteases such as SENP1, SENP2 and SENP7. Check the datasheet of any product for its validated reactivity.
Other SUMO specific peptidases from the same HGNC gene group, for researchers studying the SUMO pathway.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96HI0; product specifications from Assay Genie datasheets.