POU3F2
POU3F2 (POU class 3 homeobox 2, also known as BRN2, OCT7, OTF7 and POUF3) is a nuclear transcription factor of the POU-III class that plays a key role in neuronal differentiation. It binds DNA at a bipartite recognition sequence to regulate neuronal gene expression. Browse Assay Genie's current range of POU3F2 research reagents below, or read on for the biology behind the target.
POU3F2 is a transcription factor that plays a key role in neuronal differentiation. It binds preferentially to a recognition sequence made up of two distinct half-sites, GCAT and TAAT, separated by a non-conserved spacer of 0, 2 or 3 nucleotides. When it binds cooperatively with SOX4, SOX11 or SOX12 at gene promoters, it acts as a transcriptional activator.
The POU3F2 protein is best known in the context of cell reprogramming. The combination of three transcription factors, ASCL1, POU3F2 (BRN2) and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. POU3F2 acts downstream of ASCL1, accessing chromatin that ASCL1 has opened, and promotes transcription of neuronal genes. This makes it a useful reference point for studies of how transcription factors establish neuronal identity.
The POU3F2 gene encodes a member of the POU-III class of neural transcription factors, within the POU transcription factor family and its class-3 subfamily. According to the NCBI Gene summary, the encoded protein enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of the protein is associated with increased proliferation of melanoma cells. The protein is also listed under the names BRN2, OCT7, OTF7 and POUF3, and orthologues are present in mouse and rat (both Pou3f2).
POU3F2 plays a key role in neuronal differentiation and promotes transcription of neuronal genes.
Together with ASCL1 and MYT1L, POU3F2 is sufficient to convert fibroblasts and other somatic cells into induced neuronal cells in vitro.
POU3F2 binds a bipartite GCAT and TAAT motif and activates promoters cooperatively with SOX4, SOX11 or SOX12.
Overexpression of POU3F2 is associated with increased proliferation of melanoma cells, making it relevant to melanoma biology.
POU3F2 is a nuclear transcription factor that plays a key role in neuronal differentiation. It binds a recognition sequence of GCAT and TAAT half-sites, activates promoters with SOX4, SOX11 or SOX12, and acts downstream of ASCL1 to promote transcription of neuronal genes.
POU3F2 is the HGNC symbol for POU class 3 homeobox 2. The protein is also called POU domain, class 3, transcription factor 2, and the gene has the aliases POUF3, BRN2, OCT7 and OTF7. BRN2 is the name most often used in reprogramming literature.
The NCBI Gene summary associates POU3F2 overexpression with increased proliferation of melanoma cells. Orthologues exist in mouse and rat, both named Pou3f2. Check the datasheet of any product for its validated reactivity.
Other POU class homeobox transcription factors, including fellow POU-III members, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P20265; product specifications from Assay Genie datasheets.