NHERF2
NHERF2 (NHERF family PDZ scaffold protein 2, also known as E3KARP, SIP-1, TKA-1, NHERF-2 and OCTS2) is a PDZ domain scaffold that links plasma membrane proteins to the actin cytoskeleton. It regulates the surface expression and activity of transporters and receptors, including SLC9A3. Browse Assay Genie's current range of NHERF2 research reagents below, or read on for the biology behind the target.
NHERF2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family, and in doing so helps to tether them to the actin cytoskeleton and to regulate their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3, the sodium/hydrogen exchanger 3. UniProt also notes that it may act as a scaffold protein in the nucleus.
The NHERF2 gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The NHERF2 protein is reported in the endomembrane system, the nucleus and the apical cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, so researchers choosing an NHERF2 antibody or NHERF2 ELISA kit may wish to consider which isoforms are relevant to their work.
NHERF2 has a role in intestinal sodium absorption by regulating the activity of sodium/hydrogen exchanger 3, and it may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. The name reflects its first-described function: earlier HGNC names describe it as a regulator of solute carrier family 9 member 3, which is why the symbol SLC9A3R2 is still widely used. Mouse and rat both carry an orthologous Nherf2 gene, allowing the biology to be studied in rodent models.
NHERF2 regulates sodium/hydrogen exchanger 3 activity and contributes to intestinal sodium absorption, making it relevant to studies of epithelial transport.
By linking membrane proteins to ezrin/moesin/radixin family members and actin, NHERF2 is a model for PDZ-based organisation of the cell surface.
NHERF2 is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3, connecting second-messenger signalling to transporter control.
NHERF2 may regulate the CFTR ion channel, linking this scaffold to research on chloride channel regulation and cystic fibrosis biology.
NHERF2 is a PDZ scaffold protein that connects plasma membrane proteins with ezrin/moesin/radixin family members, linking them to the actin cytoskeleton and regulating their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3, and may also act as a scaffold in the nucleus.
NHERF2 is also called E3KARP, SIP-1, TKA-1, NHERF-2, OCTS2 and SLC9A3R2, and the UniProt name is Na(+)/H(+) exchange regulatory cofactor NHE-RF2. NCBI Gene reports that alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Mouse and rat each have an orthologue named Nherf2. In humans, NHERF2 belongs to the NHERF family of PDZ scaffold proteins, a group that regulates membrane expression of receptors and transport proteins. Check the datasheet of any product for its validated reactivity.
Other NHERF family scaffolds, ezrin/moesin/radixin proteins and transporter partners that work with NHERF2 at the cell surface.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15599; product specifications from Assay Genie datasheets.