AMOTL2
AMOTL2 (angiomotin like 2, also known as LCCP) is a member of the angiomotin family that couples actin fibres to cell junctions and restrains Wnt/beta-catenin and YAP1 signalling. It contributes to endothelial cell morphology, epithelial sheet organisation and angiogenesis. Browse Assay Genie's current range of AMOTL2 research reagents below, or read on for the biology behind the target.
AMOTL2 organises the actin cytoskeleton at cell contacts. It anchors radial actin fibres to CDH1 junction complexes at the cell membrane, which supports radial actin fibre structure and the cellular response to contractile forces. In endothelial cells it couples actin fibres to cell junctions, allowing transcellular transmission of mechanical force and the cell elongation that depends on it. Through these roles it helps maintain cell area, size, shape and epithelial sheet organisation, and contributes to trophectoderm cell properties that facilitate blastocyst zona hatching. It also regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites and selectively promotes FGF-induced MAPK activation through SRC.
AMOTL2 is a negative regulator of two growth-control pathways. It inhibits Wnt/beta-catenin signalling, probably by recruiting CTNNB1 to recycling endosomes and so preventing its translocation to the nucleus. It activates the Hippo pathway in response to cell contact inhibition: after interaction with and ubiquitination by Crumbs complex-bound WWP1, AMOTL2 interacts with LATS2, which phosphorylates YAP1 and excludes it from the nucleus, localising it to the cytoplasm and tight junctions and repressing YAP1-driven transcription. AMOTL2 also inhibits WWTR1/TAZ coactivator activity by sequestering it in the cytoplasm and at tight junctions. At myofibril neuromuscular junctions it regulates the size and protein composition of the podosome cortex and core.
The AMOTL2 gene encodes the angiomotin-like protein 2, which is related to angiomotin and belongs to the motin protein family. Angiomotin binds angiostatin, a circulating inhibitor of angiogenesis, and mediates its inhibition of endothelial cell migration and tube formation in vitro. AMOTL2 participates in angiogenesis and may play a role in the polarity, proliferation and migration of endothelial cells. Alternative splicing of the gene produces multiple transcript variants.
AMOTL2 links Crumbs-complex WWP1 and LATS2 to YAP1 phosphorylation and cytoplasmic retention, making it a point of study in contact inhibition.
AMOTL2 participates in angiogenesis and supports endothelial cell elongation, polarity, proliferation and migration.
AMOTL2 inhibits Wnt/beta-catenin signalling, probably by recruiting CTNNB1 to recycling endosomes and limiting its nuclear translocation.
AMOTL2 anchors radial actin fibres to CDH1 junctions, shaping cell morphology and the response to contractile force.
AMOTL2 links actin fibres to cell junctions, helping to maintain cell shape and epithelial sheet organisation. It also inhibits Wnt/beta-catenin signalling and activates the Hippo pathway, leading to LATS2-mediated phosphorylation of YAP1 and its exclusion from the nucleus.
The HGNC name is angiomotin like 2, the UniProt name is angiomotin-like protein 2, and LCCP is a listed alias. It belongs to the angiomotin (motin) family, and alternative splicing of the gene yields multiple transcript variants.
Orthologues named Amotl2 exist in both mouse and rat. AMOTL2 is related to angiomotin and is a member of the motin family; check the datasheet of any product for its validated reactivity.
Other motin family members and related junctional or signalling targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y2J4; product specifications from Assay Genie datasheets.