INTS8
INTS8 (integrator complex subunit 8, also known as INT8, C8orf52 and FLJ20530) is a subunit of the Integrator complex, which terminates RNA polymerase II transcription in the promoter-proximal region of genes. Within the complex, INTS8 is required for recruitment of protein phosphatase 2A (PP2A). Browse Assay Genie's current range of INTS8 research reagents below, or read on for the biology behind the target.
INTS8 is a component of the Integrator complex, a multiprotein assembly that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The complex acts as a quality checkpoint during transcription elongation, driving premature termination of transcripts that are unfavourably configured for elongation. It does so in three ways: by catalysing dephosphorylation of the C-terminal domain (CTD) of the Pol II subunit POLR2A/RPB1 and of SUPT5H/SPT5, by degrading the exiting nascent RNA through endonuclease activity, and by promoting release of Pol II from bound DNA.
Within the complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to the transcription pause-release checkpoint. The Integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Researchers studying the INTS8 protein therefore work at the intersection of transcription elongation control and non-coding RNA biology.
The INTS8 gene, formerly named chromosome 8 open reading frame 52 (C8orf52), encodes a protein of the Integrator subunit 8 family that localises to the nucleus and chromosome. NCBI Gene describes the encoded protein as associating with RNA polymerase II and being recruited to the U1 and U2 snRNA genes, where the Integrator complex is involved in cleavage of these snRNAs. Alternative splicing results in multiple transcript variants. The mouse ortholog is Ints8.
INTS8 belongs to the Integrator complex, which terminates Pol II transcription in the promoter-proximal region and acts as a checkpoint on elongation.
INTS8 is required to recruit PP2A to the pause-release checkpoint, linking Integrator to CTD and SPT5 dephosphorylation.
The Integrator complex is involved in cleaving U1 and U2 snRNAs, and INTS8 is recruited with Pol II to these genes.
The Integrator complex terminates synthesis of eRNAs, snRNAs, telomerase RNAs and lncRNAs, making INTS8 relevant to non-coding transcript control.
INTS8 is a subunit of the Integrator complex, which terminates RNA polymerase II transcription near promoters by dephosphorylating the Pol II CTD and SPT5, degrading nascent RNA and releasing Pol II from DNA. INTS8 is required to recruit PP2A to the pause-release checkpoint.
The HGNC name is integrator complex subunit 8. Aliases and previous symbols include INT8, FLJ20530, MGC131633 and C8orf52, the last reflecting the earlier name chromosome 8 open reading frame 52. NCBI Gene notes that alternative splicing produces multiple transcript variants.
A mouse ortholog, Ints8, is recorded for the human gene. UniProt places the protein in the Integrator subunit 8 family. Check the datasheet of any product for its validated reactivity before using it in a particular species.
Other Integrator complex subunits involved in Pol II transcription termination and snRNA processing, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q75QN2; product specifications from Assay Genie datasheets.