SRSF3
SRSF3 (serine and arginine rich splicing factor 3, also known as SRp20 or SFRS3) is a member of the serine/arginine-rich family of pre-mRNA splicing factors. It binds pre-mRNA to promote exon inclusion during alternative splicing and also acts as an adapter in mRNA nuclear export. Browse Assay Genie's current range of SRSF3 research reagents below, or read on for the biology behind the target.
SRSF3 is a splicing factor that binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes the inclusion of specific exons during alternative splicing. Its recruitment to binding elements adjacent to N6-methyladenosine (m6A) sites within exons is promoted by interaction with YTHDC1, an RNA-binding protein that recognises m6A-containing RNAs.
The protein also functions as an adapter in mRNA nuclear export. It binds mRNA, which is thought to be transferred to the NXF1-NXT1 heterodimer for export through the TAP/NXF1 pathway, and it enhances the RNA-binding activity of NXF1-NXT1. For m6A-containing mRNAs, interaction with YTHDC1 facilitates binding of the transcript to both SRSF3 and NXF1, promoting nuclear export. The SR proteins have also been linked to translation.
The SRSF3 gene encodes a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which form part of the spliceosome. Like other family members it contains an RNA recognition motif (RRM) for binding RNA and an RS domain, rich in serine and arginine, which binds other proteins and facilitates interaction between different SR splicing factors. UniProt lists localisation to the nucleus, nuclear speckles and cytoplasm. Two transcript variants have been described, one protein-coding and one non-coding. HGNC places SRSF3 in the RNA binding motif containing and serine and arginine rich splicing factor gene groups; earlier symbols include SFRS3 and the alias SRp20.
SRSF3 promotes inclusion of specific exons by binding a defined pre-mRNA motif, making it a useful factor for studying exon selection.
SRSF3 acts as an adapter that enhances NXF1-NXT1 RNA binding, linking it to studies of the TAP/NXF1 export pathway.
Interaction with YTHDC1 recruits SRSF3 to sites adjacent to m6A within exons and supports export of m6A-containing mRNAs.
As an SR family member with RRM and RS domains, SRSF3 helps to explain how SR proteins interact with RNA and each other.
SRSF3 is a splicing factor that binds a specific motif in pre-mRNA and promotes exon inclusion during alternative splicing. It also acts as an adapter in mRNA nuclear export, enhancing NXF1-NXT1 RNA-binding activity, including for m6A-containing mRNAs through YTHDC1.
SRSF3 is also called SRp20, and its previous gene symbol was SFRS3. Earlier names include splicing factor, arginine/serine-rich 3 and serine/arginine-rich splicing factor 3. The gene has two reported transcript variants, one protein-coding and one non-coding.
A mouse ortholog, Srsf3, is recorded for this gene. SRSF3 belongs to the SR family of splicing factors, which share an RNA recognition motif and an RS domain. Check the datasheet of any product for its validated reactivity.
Other serine/arginine-rich splicing factors and RNA-binding proteins involved in pre-mRNA processing, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P84103; product specifications from Assay Genie datasheets.