EXOSC7
EXOSC7 (exosome component 7, also known as RRP42, hRrp42p, Rrp42p, EAP1, KIAA0116 and p8) is a non-catalytic subunit of the RNA exosome, a 3'->5' exoribonuclease complex. It contributes to RNA processing and degradation in both the nucleus and the cytoplasm. Browse Assay Genie's current range of EXOSC7 research reagents below, or read on for the biology behind the target.
EXOSC7 is a non-catalytic component of the RNA exosome complex, which has 3'->5' exoribonuclease activity and takes part in a wide range of cellular RNA processing and degradation events. The catalytically inactive core of nine subunits, termed Exo-9, is proposed to bind and present RNA for ribonucleolysis and to act as a scaffold for the association of catalytic subunits and accessory proteins or complexes. EXOSC7 is part of this assembly rather than a nuclease in its own right.
In the nucleus, the RNA exosome is involved in the proper maturation of stable RNA species such as rRNA, snRNA and snoRNA. It also removes RNA processing by-products, non-coding 'pervasive' transcripts such as antisense RNAs and promoter-upstream transcripts (PROMPTs), and mRNAs with processing defects, limiting their export to the cytoplasm. The complex may also contribute to Ig class switch recombination and somatic hypermutation by targeting AICDA deamination activity to transcribed dsDNA substrates.
In the cytoplasm, the RNA exosome is involved in general mRNA turnover. It specifically degrades inherently unstable mRNAs that carry AU-rich elements in their 3' untranslated regions, and it participates in RNA surveillance pathways that prevent translation of aberrant mRNAs. It also seems to be involved in histone mRNA degradation. NCBI Gene predicts that EXOSC7 enables AU-rich region binding and places it in the cytosol, nuclear speckles and nucleolus. The EXOSC7 protein belongs to the RNase PH family and is encoded by the human EXOSC7 gene, with Exosc7 as the mouse ortholog.
EXOSC7 sits within the exosome complex that matures rRNA, snRNA and snoRNA and clears processing by-products in the nucleus.
The RNA exosome degrades unstable ARE-containing mRNAs and aberrant transcripts, making EXOSC7 relevant to studies of cytoplasmic RNA turnover.
The complex eliminates pervasive transcripts, including antisense RNAs and PROMPTs, which links EXOSC7 to work on transcriptome quality control.
The RNA exosome may help target AICDA activity during class switch recombination and somatic hypermutation, an area where EXOSC7 is of interest.
EXOSC7 is a non-catalytic subunit of the RNA exosome. As part of the Exo-9 core it is proposed to bind and present RNA for ribonucleolysis and to scaffold catalytic subunits and accessory factors, supporting RNA maturation, decay and surveillance in the nucleus and cytoplasm.
EXOSC7 stands for exosome component 7. UniProt names the protein exosome complex component RRP42, and aliases include hRrp42p, Rrp42p, RRP42, EAP1, KIAA0116 and p8. It is classed in the HGNC exosome complex gene group and belongs to the RNase PH family.
A mouse ortholog, Exosc7, is recorded for the human gene. The RNA exosome is a multi-subunit complex, and EXOSC7 is one of nine subunits in its catalytically inactive core. Check the datasheet of any product for its validated reactivity.
Other RNA exosome subunits and related RNA-processing targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15024; product specifications from Assay Genie datasheets.