APOA4
APOA4 (apolipoprotein A4, previously apolipoprotein A-IV) is a secreted apolipoprotein and a major component of HDL and chylomicrons. It is a potent activator of lecithin-cholesterol acyltransferase (LCAT) and may contribute to chylomicron and VLDL secretion and catabolism. Browse Assay Genie's current range of APOA4 research reagents below, or read on for the biology behind the target.
APOA4 encodes apolipoprotein A-IV, a lipoprotein-associated protein found in HDL and chylomicrons. UniProt annotates it as potentially having a role in the secretion and catabolism of chylomicrons and VLDL. It is required for efficient activation of lipoprotein lipase by ApoC-II, and it is a potent activator of LCAT. The NCBI Gene summary notes that, although its precise function is not known, apo A-IV activates lecithin-cholesterol acyltransferase in vitro.
The APOA4 gene contains three exons separated by two introns, and a sequence polymorphism has been identified in the 3' UTR of the third exon. The primary translation product is a 396-residue preprotein. After proteolytic processing, the mature protein is secreted from the intestine, its primary site of synthesis, in association with chylomicron particles. Researchers studying this APOA4 protein therefore work mainly with a secreted, lipoprotein-bound form.
HGNC places apolipoprotein A4 in the apolipoproteins gene group, and UniProt assigns it to the apolipoprotein A1/A4/E family. Related apolipoproteins include ApoA1, APOA2 and APOA5. The protein was previously named apolipoprotein A-IV, and the same gene is called Apoa4 in mouse and rat, which allows comparative work on lipoprotein metabolism in rodent models.
APOA4 is a major component of HDL and chylomicrons, making it a relevant protein for studying lipoprotein particle composition and turnover.
UniProt notes a possible role in chylomicron and VLDL secretion and catabolism, which makes APOA4 useful in work on triglyceride-rich lipoproteins.
APOA4 is a potent LCAT activator and is required for efficient lipoprotein lipase activation by ApoC-II, so it features in enzyme-cofactor studies.
The intestine is the primary site of synthesis, with secretion alongside chylomicron particles, linking APOA4 to studies of dietary lipid handling.
APOA4 is a secreted apolipoprotein and a major component of HDL and chylomicrons. It is required for efficient activation of lipoprotein lipase by ApoC-II, is a potent activator of LCAT, and may have a role in chylomicron and VLDL secretion and catabolism. Its precise function is not fully known.
The HGNC full name is apolipoprotein A4, and the protein was previously named apolipoprotein A-IV, also written apo A-IV or Apoa-IV. No other aliases are listed. The primary translation product is a 396-residue preprotein that is processed before secretion.
Yes. Mouse and rat both have an ortholog named Apoa4, so the gene can be studied in rodent models. APOA4 belongs to the apolipoprotein A1/A4/E family. Check the datasheet of any product for its validated reactivity.
Other apolipoproteins from the A family and related lipoprotein metabolism targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P06727; product specifications from Assay Genie datasheets.