KDM1A
KDM1A (lysine demethylase 1A, also known as LSD1, AOF2, BHC110, KIAA0601 and KDM1) is a nuclear, flavin-dependent histone demethylase of the flavin monoamine oxidase family. It removes repressive and activating methyl marks from histones and takes part in several histone deacetylase complexes. Browse Assay Genie's current range of KDM1A research reagents below, or read on for the biology behind the target.
KDM1A is a histone demethylase that acts on chromatin in the nucleus. In the neuronal context described by UniProt, it demethylates mono- and dimethylated lysine 20 of histone H4 (H4K20me1 and H4K20me2), a repressive chromatin mark. This activity is crucial for the initiation and elongation of neuronal activity-regulated genes and is required for spatial learning and memory. KDM1A also mediates H3K9me2 demethylation in cooperation with the supervillin protein (SVIL), and this H3K9 demethylase activity is essential for regulating gene expression during neuronal differentiation.
The KDM1A gene encodes a protein with a SWIRM domain, a FAD-binding motif and an amine oxidase domain, and the protein belongs to the flavin monoamine oxidase family. NCBI Gene notes that it is a component of several histone deacetylase complexes, yet it silences genes through its histone demethylase function. Alternative splicing gives rise to multiple transcript variants. HGNC places it in the lysine demethylase 1 family and among the CoREST complex subunits and flavoproteins. Mouse and rat each carry a Kdm1a ortholog, so the protein can be studied in common rodent models.
The target has been known under several names. LSD1 and AOF2 reflect its activity and its amine oxidase domain, and earlier HGNC names were amine oxidase (flavin containing) domain 2 and lysine (K)-specific demethylase 1. Researchers working with KDM1A antibody, KDM1A ELISA kit or KDM1A protein reagents will find all of these aliases in the literature.
KDM1A removes methyl groups from histone lysines, making it a central enzyme for studying how chromatin marks such as H4K20me and H3K9me2 are reversed.
Its H4K20 demethylation supports activity-regulated gene expression, and its H3K9 activity supports neuronal differentiation, linking the enzyme to learning and memory.
KDM1A is a component of several histone deacetylase complexes and a CoREST complex subunit, so it is relevant to co-repressor and gene silencing research.
With a FAD-binding motif and an amine oxidase domain, KDM1A is a flavin-dependent enzyme studied for its demethylation mechanism and cofactor requirements.
KDM1A is a nuclear histone demethylase. It demethylates mono- and dimethylated H4K20 and, with supervillin (SVIL), H3K9me2. These activities regulate neuronal activity-regulated genes and neuronal differentiation, and UniProt notes a requirement for spatial learning and memory.
KDM1A is also called LSD1, AOF2, BHC110, KIAA0601 and KDM1. Earlier HGNC names were amine oxidase (flavin containing) domain 2 and lysine (K)-specific demethylase 1. NCBI Gene reports that alternative splicing produces multiple transcript variants.
Yes. Mouse and rat both have a Kdm1a ortholog of the human gene. KDM1A belongs to the flavin monoamine oxidase family and the lysine demethylase 1 family; check the datasheet of any product for its validated reactivity.
Other lysine demethylases involved in histone methylation and epigenetic regulation.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O60341; product specifications from Assay Genie datasheets.