ATG10
ATG10 (autophagy related 10) is an E2-like enzyme of the autophagy pathway that catalyses the conjugation of ATG12 to ATG5. It is also known as APG10L, DKFZP586I0418 and FLJ13954, and belongs to the ATG10 family. Browse Assay Genie's current range of ATG10 research reagents below, or read on for the biology behind the target.
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 acts as an E2-like enzyme in this pathway, catalysing the conjugation of ATG12 to ATG5, a step that is required for autophagy. It likely serves as an ATG5-recognition molecule. The source annotation states that ATG10 is not involved in ATG12 conjugation to ATG3, which distinguishes its role from that of other components of the ubiquitin-like conjugation machinery.
According to the NCBI Gene summary, ATG10 participates in two ubiquitin-like modifications that are essential for autophagosome formation: the ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3 (MAP1LC3A), a homologue of yeast Apg8, into a membrane-bound form. The ATG10 protein is annotated as a cytoplasmic enzyme with the EC number 2.3.2.-, and the ATG10 gene is placed by HGNC in the groups autophagy related and autophagy related E2 enzymes. The mouse ortholog is Atg10.
The gene was previously named APG10 autophagy 10-like (S. cerevisiae) and ATG10 autophagy related 10 homolog (S. cerevisiae), reflecting its origin as a homologue of the yeast gene. UniProt also records a role for ATG10 in adenovirus-mediated cell lysis. Together with ATG7, ATG3, ATG5 and ATG12, ATG10 is part of the conjugation systems studied in autophagosome biogenesis.
ATG10 catalyses ATG12-ATG5 conjugation, which is required for autophagy, making it a key enzyme for studying autophagosome formation.
As an E2-like enzyme, ATG10 provides a model for how ubiquitin-like modifiers are transferred in non-ubiquitin pathways.
ATG10 contributes to the conversion of soluble MAP-LC3 to a membrane-bound form, relevant to work on autophagosome membrane biology.
UniProt annotates a role for ATG10 in adenovirus-mediated cell lysis, linking autophagy machinery to viral infection research.
ATG10 is an E2-like enzyme that catalyses the conjugation of ATG12 to ATG5, a step required for autophagy. It likely recognises ATG5 and is not involved in ATG12 conjugation to ATG3. It also contributes to the modification of MAP-LC3 to its membrane-bound form.
ATG10 is also known as APG10L, DKFZP586I0418 and FLJ13954. UniProt names the protein ubiquitin-like-conjugating enzyme ATG10. Earlier HGNC names described it as a homologue of the yeast gene, for example APG10 autophagy 10-like (S. cerevisiae).
Yes. The mouse ortholog is Atg10, and the human gene was originally named as a homologue of yeast APG10. This conservation allows autophagy conjugation to be studied across model systems; check the datasheet of any product for its validated reactivity.
Other autophagy-related enzymes, conjugation partners and pathway components with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H0Y0; product specifications from Assay Genie datasheets.