GSTK1
GSTK1 (glutathione S-transferase kappa 1, also known as GST13) is a peroxisomal enzyme of the kappa class of the glutathione transferase superfamily. It catalyses the conjugation of glutathione to exogenous and endogenous compounds as part of cellular detoxification. Browse Assay Genie's current range of GSTK1 research reagents below, or read on for the biology behind the target.
GSTK1 is a glutathione S-transferase that catalyses the conjugation of glutathione to exogenous and endogenous compounds. According to the RefSeq summary, it acts on a wide range of hydrophobic substrates, which facilitates their removal from cells. This places the enzyme within the cellular detoxification machinery. In UniProt, significant glutathione-conjugating activity of the protein is reported only with the model substrate 1-chloro-2,4-dinitrobenzene (CDNB), a point worth bearing in mind when designing activity assays.
The GSTK1 gene encodes a member of the kappa class of the glutathione transferase superfamily. The protein belongs to the GST superfamily, Kappa family, and HGNC places it in the group of soluble glutathione S-transferases. It is localised to the peroxisome. Alternative splicing of the gene results in multiple transcript variants, so researchers working with the GSTK1 protein should consider which transcript or product they wish to study. Orthologues are annotated in mouse and rat, both named Gstk1, which supports comparative work in rodent models.
The enzyme carries the EC number 2.5.1.18 and is also known by the alias GST13. Because the kappa class is distinct from other soluble glutathione transferases, GSTK1 is a useful subject for studying how glutathione conjugation is distributed between cellular compartments, particularly the peroxisome. Common approaches include a GSTK1 antibody for localisation and expression studies, and a GSTK1 ELISA kit for quantification, alongside activity measurements with CDNB.
GSTK1 conjugates glutathione to hydrophobic compounds, facilitating their removal from cells, so it is relevant to studies of xenobiotic and endogenous compound handling.
The protein is localised to the peroxisome, making it a candidate marker for glutathione-dependent processes within this organelle.
Significant conjugating activity is reported with CDNB, providing a model substrate for characterising kappa class glutathione S-transferase activity.
As the kappa family member of the GST superfamily, GSTK1 supports comparisons with other soluble glutathione S-transferases and across mouse and rat orthologues.
GSTK1 is a glutathione S-transferase that catalyses the conjugation of glutathione to exogenous and endogenous compounds. It acts on hydrophobic substrates and helps remove them from cells. Significant activity is reported only with the model substrate CDNB. The protein is found in the peroxisome.
GSTK1 is the HGNC symbol for glutathione S-transferase kappa 1, and GST13 is a listed alias. The enzyme has the EC number 2.5.1.18. NCBI Gene reports that alternative splicing produces multiple transcript variants, so check which variant a reagent recognises.
Yes. Mouse and rat orthologues are both annotated as Gstk1, so the gene can be studied in common rodent models. Sequence conservation and cross-reactivity vary between reagents, so check the datasheet of any product for its validated reactivity.
Other soluble glutathione S-transferases and detoxification enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y2Q3; product specifications from Assay Genie datasheets.