MAPRE1
MAPRE1, also known as EB1 (microtubule associated protein RP/EB family member 1), is a plus-end tracking protein that binds the growing ends of microtubules and regulates the dynamics of the microtubule cytoskeleton. It also recruits other plus-end tracking proteins to microtubules. Browse Assay Genie's current range of MAPRE1 research reagents below, or read on for the biology behind the target.
MAPRE1 is a plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates microtubule cytoskeleton dynamics. It recruits other +TIP proteins by binding a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains, and it promotes cytoplasmic microtubule nucleation and elongation. In mitosis it helps position the spindle by stabilising microtubules and promoting a dynamic connection between astral microtubules and the cortex during chromosome segregation. It also assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilising its interactions with microtubule bundles (K-fibres).
The protein also regulates minus-end microtubule organisation. It interacts with the complex formed by AKAP9 and PDE4DIP, which recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules there, a step important for polarised cell movement. MAPRE1 promotes elongation of CAMSAP2-decorated minus-end microtubule stretches and regulates autophagosome transport through CAMSAP2. It acts downstream of Rho GTPases and DIAPH1 in stable microtubule formation, promotes microtubule-mediated trafficking of GJA1/CX43 to the cell membrane through its interaction with DSP, and may play a role in cell migration. UniProt lists localisation to the cytoskeleton, centrosome, spindle, spindle pole, Golgi apparatus and cell membrane.
The MAPRE1 gene was first identified through the binding of its product to the APC protein, which is often mutated in familial and sporadic colorectal cancer. In interphase cells the protein localises to microtubules, especially the growing ends. During mitosis it associates with centrosomes and spindle microtubules. It also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein, and is thought to contribute to the regulation of microtubule structures and chromosome stability. MAPRE1 belongs to the MAPRE (RP/EB) family, and the mouse and rat orthologues are both called Mapre1.
MAPRE1 tracks growing microtubule plus-ends, regulates cytoskeleton dynamics and promotes microtubule nucleation and elongation.
It supports spindle positioning and chromosome alignment in metaphase, including recruitment of the SKA complex to K-fibres.
Through CAMSAP2, MAPRE1 tethers non-centrosomal minus-end microtubules to the Golgi, a step important for polarised cell movement.
MAPRE1 contributes to autophagosome transport and to microtubule-mediated GJA1/CX43 delivery to the membrane, supporting gap junction communication.
MAPRE1 is a plus-end tracking protein that binds microtubule plus-ends and regulates cytoskeleton dynamics. It recruits other +TIP proteins through their SXLP motif, promotes microtubule nucleation and elongation, and supports mitotic spindle positioning and chromosome alignment in metaphase.
Yes. EB1 is an alias for MAPRE1, whose full name is microtubule associated protein RP/EB family member 1. The protein was first identified by its binding to APC. It belongs to the MAPRE family, alongside related members such as MAPRE3.
Mouse and rat both have an orthologous gene named Mapre1, so the target can be studied in common rodent models. Reactivity differs between reagents, so check the datasheet of any product for its validated reactivity.
Other members of the MAPRE family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15691; product specifications from Assay Genie datasheets.