SF1
SF1 (splicing factor 1) is a nuclear pre-mRNA splicing factor that recognises the intron branch point sequence and is needed for early spliceosome assembly. It is also known as ZFM1, ZCCHC25, BBP and ZNF162. Browse Assay Genie's current range of SF1 research reagents below, or read on for the biology behind the target.
SF1 is necessary for the ATP-dependent first step of spliceosome assembly. It binds the intron branch point sequence (BPS) of the pre-mRNA, 5'-UACUAAC-3', and recognises this element at the 3' splice site together with the large subunit of U2 auxiliary factor (U2AF). Beyond splicing, the encoded protein plays a role in nuclear pre-mRNA retention and transcriptional repression, and UniProt notes it may act as a transcription repressor.
The SF1 protein has a modular architecture: an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region that together bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. It is a nuclear protein and belongs to the BBP/SF1 family. Alternative splicing of the SF1 gene results in multiple transcript variants. The mouse ortholog is Sf1.
HGNC classifies SF1 within the CCHC-type zinc fingers, and within the spliceosomal A and E complex gene groups. Its history is reflected in its aliases: the gene was previously named zinc finger protein 162 (ZNF162), and it is also listed as ZFM1, ZCCHC25 and BBP. These names are useful to know when searching the literature on branch point recognition and early spliceosome assembly.
SF1 is required for the ATP-dependent first step of spliceosome assembly, making it a key subject for work on early splicing complexes.
SF1 binds the intron branch point sequence at the 3' splice site, alongside U2AF, and helps define how branch sites are recognised.
Its hnRNP K homology motif, quaking 2 region and zinc knuckles make SF1 a model for studying how modular domains engage RNA.
SF1 contributes to nuclear pre-mRNA retention and transcriptional repression, linking splicing machinery to wider control of gene expression.
SF1 is necessary for the ATP-dependent first step of spliceosome assembly. It binds the intron branch point sequence 5'-UACUAAC-3' of the pre-mRNA and, with the large subunit of U2AF, recognises the branch point at the 3' splice site. It also contributes to nuclear pre-mRNA retention and transcriptional repression.
SF1 is also called ZFM1, ZCCHC25, BBP and ZNF162, and its previous name was zinc finger protein 162. Alternative splicing of the SF1 gene produces multiple transcript variants. Check the datasheet of any product for its validated reactivity.
A mouse ortholog, Sf1, is recorded for this gene. UniProt places the protein in the BBP/SF1 family, and the BBP alias reflects its role as a branch point binding protein. Check the datasheet of any product for its validated reactivity.
Other splicing factors and similarly named proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15637; product specifications from Assay Genie datasheets.