GMPS
GMPS (guanosine monophosphate synthase, also known as GATD7 and formerly guanine monophosphate synthetase) is a cytosolic enzyme in guanine nucleotide biosynthesis. It converts xanthine monophosphate (XMP) to GMP using glutamine and ATP. Browse Assay Genie's current range of GMPS research reagents below, or read on for the biology behind the target.
GMPS catalyses the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP, proceeding through an adenyl-XMP intermediate. Because the reaction uses glutamine as the amino donor, the enzyme is described as glutamine-hydrolysing, and its UniProt name is GMP synthase [glutamine-hydrolyzing]. The enzyme is assigned EC 6.3.5.2 and is found in the cytosol.
In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which the pathway diverges towards either guanine or adenine nucleotides. Two enzymes convert IMP to GMP along the guanine branch. IMP dehydrogenase (IMPD1) catalyses the oxidation of IMP to XMP, and GMP synthetase then catalyses the amination of XMP to GMP. GMPS therefore carries out the last committed step in making guanine nucleotides from IMP.
The human GMPS gene is the subject of HGNC:4378 and encodes the GMPS protein. HGNC places it in the glutamine amidotransferase class 1 domain containing group, and lists GATD7 as an alias. An earlier approved name was guanine monophosphate synthetase. Orthologues are annotated in mouse and rat, both named Gmps, which allows the enzyme to be studied in common rodent models. Researchers working on purine metabolism, nucleotide supply and the guanine branch of the pathway use GMPS as a defined point of study.
GMPS acts after the IMP branch point in de novo purine synthesis, making it a key enzyme for studying guanine nucleotide production.
With IMPD1, GMPS defines the guanine arm of the pathway that diverges from IMP, supporting work on how nucleotide pools are balanced.
GMPS uses glutamine and ATP and forms an adenyl-XMP intermediate, offering a model for glutamine-dependent amination reactions.
Mouse and rat both carry a Gmps orthologue, so comparative studies of guanine nucleotide synthesis can span human and rodent systems.
GMPS catalyses the amination of xanthine monophosphate (XMP) to GMP, using glutamine and ATP and passing through an adenyl-XMP intermediate. It acts in the guanine nucleotide branch of de novo purine synthesis, downstream of IMP dehydrogenase (IMPD1), and is located in the cytosol.
GMPS is the HGNC symbol for guanosine monophosphate synthase. GATD7 is a listed alias, and the earlier approved name was guanine monophosphate synthetase. UniProt names the protein GMP synthase [glutamine-hydrolyzing], and the enzyme classification is EC 6.3.5.2.
Yes. Both mouse and rat have an orthologue named Gmps, so the enzyme can be studied across human and rodent systems. Check the datasheet of any product for its validated reactivity before choosing a reagent for a given species.
Other purine metabolism enzymes and nucleotide synthesis targets that sit alongside GMPS in the guanine pathway.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P49915; product specifications from Assay Genie datasheets.