RPS6
RPS6 (ribosomal protein S6, also known as S6 or eS6) is a component of the 40S small ribosomal subunit. It helps control cell growth and proliferation through the selective translation of particular classes of mRNA. Browse Assay Genie's current range of RPS6 research reagents below, or read on for the biology behind the target.
Ribosomes are the organelles that catalyse protein synthesis. They consist of a small 40S subunit and a large 60S subunit, built from four RNA species and approximately 80 structurally distinct proteins. RPS6 is a component of the 40S subunit. It plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
The RPS6 gene encodes a cytoplasmic ribosomal protein that belongs to the S6E family, described by UniProt as the eukaryotic ribosomal protein eS6 family. The protein is found in the cytoplasm and in the nucleolus. In the nucleolus it is part of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit. During SSU processome assembly, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA. Together they generate RNA folding, modifications, rearrangements and cleavage, and they target pre-ribosomal RNA for degradation by the RNA exosome.
RPS6 is the major substrate of protein kinases in the ribosome. Subsets of five C-terminal serine residues are phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumour-promoting agents and mitogens, and dephosphorylation occurs at growth arrest. This makes phospho-S6 a widely used readout of growth-related signalling. As is typical for ribosomal protein genes, the RPS6 gene has multiple processed pseudogenes dispersed through the genome. HGNC places it in the S ribosomal proteins gene group.
RPS6 is part of the SSU processome in the nucleolus, which assembles the precursor of the small eukaryotic ribosomal subunit.
RPS6 contributes to the selective translation of particular classes of mRNA, a mechanism linked to the control of cell growth.
C-terminal serine phosphorylation of RPS6 is induced by growth factors, tumour-promoting agents and mitogens, and reverses at growth arrest.
RPS6 helps control cell growth and proliferation, so its expression and phosphorylation state are relevant to proliferation research.
RPS6 is a component of the 40S small ribosomal subunit. It plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. It is also part of the SSU processome during small subunit assembly in the nucleolus.
RPS6 is the HGNC symbol for ribosomal protein S6. It is also called S6 and eS6, and UniProt names it small ribosomal subunit protein eS6. The RPS6 gene is distinct from the RPS6K genes, which encode kinases that act on it.
Mouse and rat both have an Rps6 ortholog; check the datasheet of any product for its validated reactivity. RPS6 is the major kinase substrate in the ribosome, with five C-terminal serines phosphorylated by different protein kinases in response to growth stimuli.
Other 40S ribosomal proteins and the RPS6 kinase family, which are relevant to ribosome biogenesis and growth signalling research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P62753; product specifications from Assay Genie datasheets.