ACO2
ACO2 (aconitase 2, also known as ACONM) is a mitochondrial enzyme that catalyses the isomerisation of citrate to isocitrate via cis-aconitate. It belongs to the aconitase/IPM isomerase family and acts in the second step of the TCA cycle. Browse Assay Genie's current range of ACO2 research reagents below, or read on for the biology behind the target.
ACO2 catalyses the interconversion of citrate and isocitrate, with cis-aconitate as the intermediate. This reaction is the second step of the TCA cycle, which makes the enzyme a core component of mitochondrial energy metabolism. Its systematic activity is classed as aconitate hydratase (EC 4.2.1.3), and UniProt lists the protein name as aconitate hydratase, mitochondrial.
The ACO2 gene is encoded in the nucleus, and the protein functions in the mitochondrion. It belongs to the aconitase/IPM isomerase family and is placed by HGNC in the aconitases gene group, alongside ACO1, the cytosolic aconitase. Mouse and rat both carry an orthologous Aco2 gene, so the enzyme can be studied in common rodent models.
HGNC records the approved name as aconitase 2, with the previous name aconitase 2, mitochondrial and the alias ACONM. According to the NCBI Gene summary, the ACO2 protein is one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease PRSS15, also known as Lon protease, after oxidative modification. This link between oxidative modification and protein turnover makes ACO2 a useful subject for work on mitochondrial protein quality control and redox biology. Researchers working on the TCA cycle, mitochondrial function or oxidative stress may therefore look at ACO2 protein levels alongside other metabolic enzymes.
ACO2 catalyses the conversion of citrate to isocitrate via cis-aconitate, the second step of the TCA cycle.
The enzyme is encoded in the nucleus but functions in the mitochondrion, making it a reference protein for the mitochondrial matrix.
ACO2 is among the matrix proteins preferentially degraded by Lon protease after oxidative modification.
Its degradation by PRSS15 (Lon protease) links ACO2 to the handling of damaged proteins within the mitochondrion.
ACO2 is a mitochondrial enzyme that catalyses the isomerisation of citrate to isocitrate via cis-aconitate. This is the second step of the TCA cycle. The protein is encoded in the nucleus and carries out its function inside the mitochondrion.
HGNC lists the approved name as aconitase 2, with the previous name aconitase 2, mitochondrial and the alias ACONM. UniProt names the protein aconitate hydratase, mitochondrial, and its enzyme classification is EC 4.2.1.3.
Yes. Mouse and rat both have an orthologous gene named Aco2. ACO2 belongs to the aconitase/IPM isomerase family, and HGNC groups it with the aconitases, which also include ACO1. Check the datasheet of any product for its validated reactivity.
Other aconitase family members and related metabolic enzymes with research reagents at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q99798; product specifications from Assay Genie datasheets.