NOTCH2
NOTCH2 (notch receptor 2) is a type 1 transmembrane receptor of the Notch family that binds membrane-bound ligands to regulate cell-fate determination. Its signal is carried into the nucleus by the notch intracellular domain, where it drives transcription of target genes. Browse Assay Genie's current range of NOTCH2 research reagents below, or read on for the biology behind the target.
NOTCH2 functions as a receptor for the membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1). Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Through this route NOTCH2 affects the implementation of differentiation, proliferation and apoptotic programmes.
The NOTCH2 gene encodes a member of the Notch family, whose members are type 1 transmembrane proteins. The extracellular region consists of multiple epidermal growth factor-like (EGF) repeats, while the intracellular region contains several different domain types. The protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. Two transcript variants encoding different isoforms have been described. UniProt lists localisation to the cell membrane, nucleus and cytoplasm, consistent with a receptor whose intracellular domain moves to the nucleus after activation.
The Notch signalling network is an evolutionarily conserved intercellular pathway that regulates interactions between physically adjacent cells, and Notch proteins have a role in many developmental processes. NOTCH2 may play a role in vascular, renal and hepatic development. It is also involved in bone remodelling and homeostasis: together with RELA/p65 it enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. It also positively regulates self-renewal of liver cancer cells. Earlier names for the gene include notch 2 and Notch homolog 2 (Drosophila).
NOTCH2 controls cell-fate decisions and may contribute to vascular, renal and hepatic development, making it a focus for studies of differentiation.
Ligand binding by JAG1, JAG2 and DLL1 releases NICD, which partners with RBPJ to activate enhancer of split genes.
NOTCH2 is involved in bone remodelling and, with RELA/p65, promotes NFATc1 activity and RANKL-induced osteoclast differentiation.
NOTCH2 positively regulates self-renewal of liver cancer cells, linking Notch signalling to proliferative and apoptotic programmes.
NOTCH2 is a cell-surface receptor for JAG1, JAG2 and DLL1. After ligand activation its intracellular domain forms a transcriptional activator complex with RBPJ/RBPSUH, switching on enhancer of split genes and shaping differentiation, proliferation and apoptosis. It also contributes to bone remodelling.
The HGNC name is notch receptor 2, and UniProt calls the protein neurogenic locus notch homolog protein 2. Older names include Notch homolog 2 (Drosophila) and notch 2. NCBI Gene reports two transcript variants encoding different isoforms.
Yes. The Notch signalling network is evolutionarily conserved, and mouse and rat both have a Notch2 ortholog. In Drosophila, Notch interacts with the cell-bound ligands Delta and Serrate. Check the datasheet of any product for its validated reactivity.
Other Notch receptors, their ligands and transcriptional partners in the Notch signalling pathway.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q04721; product specifications from Assay Genie datasheets.