RPS14
RPS14 (ribosomal protein S14, also known as EMTB, S14 and uS11) is a component of the 40S small ribosomal subunit, the part of the ribosome involved in cellular protein synthesis. It also takes part in the assembly of the small subunit processome in the nucleolus. Browse Assay Genie's current range of RPS14 research reagents below, or read on for the biology behind the target.
RPS14 is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Mammalian ribosomes consist of a small 40S subunit and a large 60S subunit, which together contain four RNA species and approximately 80 structurally distinct proteins. RPS14 is one of the proteins of the 40S subunit.
RPS14 is also part of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit. During SSU processome assembly in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA. Together they generate RNA folding, modifications, rearrangements and cleavage, as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. UniProt records the protein in the cytoplasm and in the nucleolus of the nucleus.
The RPS14 gene encodes a protein of the S11P family, designated uS11 in the universal ribosomal protein nomenclature, and HGNC places it in the S ribosomal proteins group. Transcript variants that use alternative transcription initiation sites have been described, and several alternatively spliced variants encode the same protein. As is typical for ribosomal protein genes, multiple processed pseudogenes are dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can confer resistance to emetine, a protein synthesis inhibitor. Mouse and rat both have an Rps14 ortholog.
RPS14 is a 40S subunit protein, so it is relevant to studies of how the small ribosomal subunit is built and organised.
RPS14 is part of the SSU processome, which assembles in the nucleolus with pre-rRNA and many biogenesis factors.
As a component of the machinery that synthesises proteins, RPS14 is relevant to work on translation in the cell.
Mutations in the RPS14 gene can cause emetine resistance in Chinese hamster ovary cells, linking it to studies of protein synthesis inhibitors.
RPS14 is a component of the 40S small ribosomal subunit, and the ribosome carries out protein synthesis in the cell. It is also part of the SSU processome, the first precursor of the small subunit, which assembles in the nucleolus.
RPS14 is the HGNC symbol for ribosomal protein S14. Aliases include EMTB, S14 and uS11. UniProt names the protein small ribosomal subunit protein uS11, reflecting the universal ribosomal protein nomenclature. Check the datasheet of any product for its validated reactivity.
Yes. RPS14 belongs to the universal ribosomal protein uS11 family, and mouse and rat both carry an Rps14 ortholog. In Chinese hamster ovary cells, mutations in this gene can lead to emetine resistance. Check the datasheet of any product for its validated reactivity.
Other small subunit ribosomal proteins and ribosome-related targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P62263; product specifications from Assay Genie datasheets.