RAD51D
RAD51D (RAD51 paralog D, also known as R51H3, Trad, HsTRAD or RAD51L3) is a member of the RAD51 protein family that takes part in homologous recombination repair of double-stranded DNA breaks. It binds single-stranded DNA, has DNA-dependent ATPase activity and contributes to telomere maintenance. Browse Assay Genie's current range of RAD51D research reagents below, or read on for the biology behind the target.
RAD51D is involved in the homologous recombination repair (HRR) pathway, which repairs double-stranded DNA breaks that arise during DNA replication or are induced by DNA-damaging agents. The protein binds single-stranded DNA and has DNA-dependent ATPase activity. It is part of the RAD51 paralog complex BCDX2, which acts in the BRCA1-BRCA2-dependent homologous recombination pathway. After DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment.
BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to the junction of replication forks. It was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and, specifically, nicks in duplex DNA. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM. RAD51D is also involved in telomere maintenance. NCBI Gene notes that the complex formed with this protein has been shown to catalyse homologous pairing between single- and double-stranded DNA, and is thought to act in the early stage of recombinational repair.
The RAD51D gene encodes a member of the RecA family, RAD51 subfamily, which is highly similar to bacterial RecA and to Saccharomyces cerevisiae Rad51. UniProt lists localisation to the nucleus, the centrosome and telomeres. The protein forms a complex with other RAD51 family members, including RAD51L1, RAD51L2 and XRCC2. Alternative splicing produces multiple transcript variants, and read-through transcription exists between this gene and the downstream RFFL gene. Earlier names include RAD51-like 3 and RAD51 homolog D. The mouse ortholog is Rad51d.
RAD51D acts in the BRCA1-BRCA2-dependent pathway that repairs double-stranded DNA breaks, downstream of BRCA2 and upstream of RAD51 recruitment.
The BCDX2 complex binds Holliday junctions and replication fork junctions, and XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
RAD51D is involved in telomere maintenance and localises to chromosome telomeres, linking recombination proteins to chromosome end function.
RAD51D partners with other RAD51 family members, including XRCC2, making it a useful subject for studying paralog complex assembly and function.
RAD51D is involved in homologous recombination repair of double-stranded DNA breaks. It binds single-stranded DNA, has DNA-dependent ATPase activity and belongs to the BCDX2 complex, which acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. It is also involved in telomere maintenance.
RAD51D is the symbol for RAD51 paralog D. Aliases include R51H3, Trad, HsTRAD and RAD51L3. UniProt names the protein DNA repair protein RAD51 homolog 4. Earlier names include RAD51-like 3 and RAD51 homolog D. Alternative splicing gives multiple transcript variants.
RAD51D belongs to the RecA family and is highly similar to bacterial RecA and yeast Rad51. The mouse ortholog is Rad51d. Researchers working in other species should check the datasheet of any product for its validated reactivity.
Other RAD51 family members and RAD-named DNA repair and checkpoint proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O75771; product specifications from Assay Genie datasheets.